Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000473
UniProt IDP45973
Primary gene name(s)CBX5
Synonym gene name(s)HP1A
Protein nameChromobox protein homolog 5
Protein functionComponent of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9', H3K9me, leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated, H3Y41ph. Can interact with lamin-B receptor, LBR. This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. {ECO:0000269|PubMed:19783980}.
Subcellular locationNucleus {ECO:0000269|PubMed:10460410}. Chromosome {ECO:0000269|PubMed:10460410}. Chromosome, centromere {ECO:0000269|PubMed:10460410, ECO:0000269|PubMed:21346195}. Note=Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P45973
Gene Ontology
(Biological Process)
Complete annatation
blood coagulation [GO:0007596];
negative regulation of transcription, DNA-templated [GO:0045892];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation
chromatin binding [GO:0003682];
methylated histone binding [GO:0035064];
protein binding, bridging [GO:0030674];
repressing transcription factor binding [GO:0070491]
Gene Ontology
(Cellular Component)
Complete annatation
chromocenter [GO:0010369];
histone deacetylase complex [GO:0000118];
histone methyltransferase complex [GO:0035097];
kinetochore [GO:0000776];
nuclear chromosome, telomeric region [GO:0000784];
nuclear envelope [GO:0005635];
nuclear heterochromatin [GO:0005720];
nuclear pericentric heterochromatin [GO:0031618];
nucleolus [GO:0005730];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
pericentric heterochromatin [GO:0005721];
transcriptional repressor complex [GO:0017053]
Protein-protein interaction117030
Phylogenetic treeP45973
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.3314213826191980.3114581447200520.430827565824213
AZA vs. DISU-0.199501947827590.4324653611479920.915650781529304
AZA vs. IL70.1969264310767640.3057016386776830.999311006273513
AZA vs. SAHA-0.2211695028431840.364840267879460.732206390362799
DISU vs. CD3-0.5425669604146110.1386342773904920.24100716150205
DISU vs. IL70.3877980589358070.1552665925116340.510918028024529
DISU vs. SAHA-0.02165636676257810.9413723731624370.98623683668783
DMSO vs. AZA-0.1304196202384080.4358502317904971
DMSO vs. CD3-0.4724264457337330.1400915066279590.224786997099333
DMSO vs. DISU0.06741250285646990.7831674659244960.972506073787257
DMSO vs. IL70.3346274241940130.06289789432149350.481005866144164
DMSO vs. SAHA-0.09886284380612380.6751002808940.897786781843686
HIV vs. Mock in Activation-0.04041948790682320.9488203264265460.999983755607037
HIV vs. Mock in Latency0.06755616629907240.6816252774124050.999834320637052
IL7 vs. CD3-0.1256680398655450.6962373924246070.794126841923276
SAHA vs. CD3-0.5789120275216680.1019211362493160.176607051083958
SAHA vs. IL7-0.4225956021580110.08429637898232310.258908255870644
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.45949 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.021 0.989 1.12 1.083 1.104
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3FDT X-ray 2.0Å A=18-75.
3I3C X-ray 2.4Å A/B/C/D=110-173.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat inactivates 12692243
23803414
Vpr relocalizes 21875947
21875947

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found
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