Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000472
UniProt IDQ13185
Primary gene name(s)CBX3
Synonym gene name(s)unknown
Protein nameChromobox protein homolog 3
Protein functionSeems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor, LBR. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1.
Subcellular locationNucleus {ECO:0000305}. Note=Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis, Potential. {ECO:0000305}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q13185
Gene Ontology
(Biological Process)
Complete annatation
cellular response to dexamethasone stimulus [GO:0071549];
chromatin remodeling [GO:0006338];
covalent chromatin modification [GO:0016569];
negative regulation of transcription, DNA-templated [GO:0045892];
rhythmic process [GO:0048511];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
enzyme binding [GO:0019899];
histone methyltransferase binding [GO:1990226];
identical protein binding [GO:0042802];
protein domain specific binding [GO:0019904]
Gene Ontology
(Cellular Component)
Complete annatation
chromatin [GO:0000785];
chromosome, centromeric region [GO:0000775];
condensed chromosome, centromeric region [GO:0000779];
nuclear chromosome, telomeric region [GO:0000784];
nuclear envelope [GO:0005635];
nuclear euchromatin [GO:0005719];
nuclear heterochromatin [GO:0005720];
nuclear inner membrane [GO:0005637];
nuclear pericentric heterochromatin [GO:0031618];
nucleus [GO:0005634];
senescence-associated heterochromatin focus [GO:0035985];
spindle [GO:0005819]
Protein-protein interaction116463
Phylogenetic treeQ13185
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.9532814725205140.004359314925539870.0129443720692495
AZA vs. DISU-0.2191085631421390.3859110365840690.900638308680944
AZA vs. IL70.1796736839024580.3487572957203270.999311006273513
AZA vs. SAHA-0.002337164694246570.9923584054768090.998263968272755
DISU vs. CD3-1.18512272214120.001347881635371690.00535528934412043
DISU vs. IL70.3898396711295880.1216238089348870.456657265178934
DISU vs. SAHA0.2174296464344040.4573976924593680.7971739618122
DMSO vs. AZA-0.09226632079799810.5806517764446461
DMSO vs. CD3-1.057031064418120.001297721287166540.00428825865127527
DMSO vs. DISU0.1250175791923270.6084229593570070.939289650701039
DMSO vs. IL70.2790457746016320.1201048670448770.611275767341979
DMSO vs. SAHA0.08265385683362790.7255565282475090.919204979591309
HIV vs. Mock in Activation-0.3726279365820360.601005681684130.999983755607037
HIV vs. Mock in Latency-0.05124073313183660.755375687828070.999834320637052
IL7 vs. CD3-0.7647626712623970.02305323361608750.0586138269204111
SAHA vs. CD3-0.9809015469284450.007356964274382150.0198096099351589
SAHA vs. IL7-0.1857866494152140.4461696779048140.685093381352467
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0971587 0.546545
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.013 0.967 0.792 0.754 0.854
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2L11 NMR - A=29-81.
3DM1 X-ray 2.4Å A/C/E/G=29-86.
3KUP X-ray 1.7Å A/B/C/D=110-173.
3TZD X-ray 1.8Å A=29-81.
5T1I X-ray 1.6Å A/B=110-176.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat upregulates 23166591
Vpr relocalizes 21875947

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found