Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000462
UniProt IDO14936
Primary gene name(s)CASK
Synonym gene name(s)LIN2
Protein namePeripheral plasma membrane protein CASK
Protein functionMultidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TBR1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1.
Subcellular locationNucleus {ECO:0000250}. Cytoplasm {ECO:0000250}. Cell membrane {ECO:0000250};
Peripheral membrane protein {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O14936
Gene Ontology
(Biological Process)
Complete annatation
calcium ion import [GO:0070509];
cell adhesion [GO:0007155];
negative regulation of cell-matrix adhesion [GO:0001953];
negative regulation of cellular response to growth factor stimulus [GO:0090288];
negative regulation of keratinocyte proliferation [GO:0010839];
negative regulation of wound healing [GO:0061045];
neurotransmitter secretion [GO:0007269];
positive regulation of calcium ion import [GO:0090280];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
guanylate kinase activity [GO:0004385];
protein serine/threonine kinase activity [GO:0004674]
Gene Ontology
(Cellular Component)
Complete annatation
actin cytoskeleton [GO:0015629];
basement membrane [GO:0005604];
basolateral plasma membrane [GO:0016323];
cell-cell junction [GO:0005911];
ciliary membrane [GO:0060170];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
focal adhesion [GO:0005925];
nuclear lamina [GO:0005652];
nuclear matrix [GO:0016363];
nucleolus [GO:0005730];
plasma membrane [GO:0005886];
presynaptic membrane [GO:0042734];
vesicle [GO:0031982]
Protein-protein interaction114141
Phylogenetic treeO14936
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.1544976855776940.6377450133279510.736065621344568
AZA vs. DISU0.01379751341012370.9566896675059420.996649735504266
AZA vs. IL7-0.04302012249664890.8244560290682550.999311006273513
AZA vs. SAHA0.1350037850041610.5817555335189760.861094158706309
DISU vs. CD30.1548636436953250.6699778182795790.766583590154538
DISU vs. IL7-0.06576451525679080.7951225650974820.954423799099071
DISU vs. SAHA0.1226617906978640.674550094019480.901653611260983
DMSO vs. AZA0.006263708045269410.9705169930362431
DMSO vs. CD30.148871818669120.6422018108081010.732604140528853
DMSO vs. DISU-0.009414802271231070.9693455652216560.995077522347534
DMSO vs. IL7-0.04192847778547650.8172719920131160.962956477091318
DMSO vs. SAHA0.122096708261150.6060775602213490.867774549695606
HIV vs. Mock in Activation0.3487811817917470.5760508892911390.999983755607037
HIV vs. Mock in Latency-0.01271956400794590.9390304302976760.999834320637052
IL7 vs. CD30.118754836622780.7121097186830490.805404801508937
SAHA vs. CD30.2643080761059830.4553238268466630.567789000461664
SAHA vs. IL70.1737508300167630.4776958017102190.710767595990508
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.167964 0.423321
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB01942 Formic Acid experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1KGD X-ray 1.3Å A=739-914.
1KWA X-ray 1.9Å A/B=487-572.
1ZL8 NMR - B=403-456.
3C0G X-ray 2.1Å A/B=1-337.
3C0H X-ray 2.3Å A/B=1-337.
3C0I X-ray 1.8Å A=1-337.
3MFR X-ray 2.0Å A=1-337.
3MFS X-ray 2.1Å A=1-337.
3MFT X-ray 2.2Å A=1-337.
3MFU X-ray 2.3Å A=1-337.
3TAC X-ray 2.2Å A=1-345.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found