Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000452
UniProt IDP40121
Primary gene name(s)CAPG
Synonym gene name(s)AFCP, MCP
Protein nameMacrophage-capping protein
Protein functionCalcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA.
Subcellular locationNucleus {ECO:0000269|PubMed:17081065, ECO:0000269|PubMed:18266911}. Cytoplasm {ECO:0000269|PubMed:17081065}. Secreted {ECO:0000250|UniProtKB:P24452}. Melanosome {ECO:0000269|PubMed:17081065}. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV, PubMed:17081065. Redistributes throughout the cytoplasm during mitosis and is excluded from regions containing chromosomes, By similarity. {ECO:0000250|UniProtKB:P24452, ECO:0000269|PubMed:17081065}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P40121
Gene Ontology
(Biological Process)
Complete annatation
barbed-end actin filament capping [GO:0051016];
protein complex assembly [GO:0006461]
Gene Ontology
(Molecular Function)
Complete annatation
protein complex binding [GO:0032403];
protein domain specific binding [GO:0019904]
Gene Ontology
(Cellular Component)
Complete annatation
centriole [GO:0005814];
cytoplasm [GO:0005737];
extracellular exosome [GO:0070062];
F-actin capping protein complex [GO:0008290];
Flemming body [GO:0090543];
melanosome [GO:0042470];
mitotic spindle [GO:0072686];
nucleolus [GO:0005730];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction107272
Phylogenetic treeP40121
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-2.836066377705853.97844668365011e-062.6973975895625e-05
AZA vs. DISU-0.2073354127699250.575719656157290.954304256505839
AZA vs. IL70.09068634388945960.6362372379124580.999311006273513
AZA vs. SAHA-0.6358177673300020.009391083445571820.0938734694022213
DISU vs. CD32.616584952117320.0002733977152841180.00136282974874101
DISU vs. IL70.288390185024040.4147239848711420.773375070748697
DISU vs. SAHA-0.4260257036474920.2437295257406510.622034267636486
DMSO vs. AZA0.04829708873226080.7802026179698921
DMSO vs. CD32.87158031656071.80520722770083e-061.22667780267537e-05
DMSO vs. DISU0.2534244606687530.472522274459640.897275884208221
DMSO vs. IL70.04988012461893130.7808056641946950.954555156022505
DMSO vs. SAHA-0.6889157817485360.003584500815876250.0437253832122961
HIV vs. Mock in Activation0.08756310284561520.9421617908510430.999983755607037
HIV vs. Mock in Latency-0.06526851523370420.7041212475240390.999834320637052
IL7 vs. CD32.93275267846229.38321516485985e-079.21989583963054e-06
SAHA vs. CD32.177239260849050.0002288226320357190.000992332968281521
SAHA vs. IL7-0.7283166593637060.002879638289661650.0257154428781751
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
1.7 0.000929725 1.3 0.033703843 1.6 0.039586016
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.390991 0.00493672
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.027 1.735 3.191 4.2 4.495
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1J72 X-ray 2.5Å A=1-348.
1JHW X-ray 2.8Å A=1-348.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpr downregulates 23874603
Tat interacts with 19454010

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found