Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000450
UniProt IDP27824
Primary gene name(s)CANX
Synonym gene name(s)unknown
Protein nameCalnexin
Protein functionCalcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.
Subcellular locationEndoplasmic reticulum membrane {ECO:0000269|PubMed:22314232};
Single-pass type I membrane protein {ECO:0000255}. Endoplasmic reticulum {ECO:0000269|PubMed:22314232}. Melanosome {ECO:0000269|PubMed:12643545, ECO:0000269|PubMed:17081065}. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV, PubMed:12643545, PubMed:17081065. The palmitoylated form preferentially localizes to the perinuclear rough ER, PubMed:22314232. {ECO:0000269|PubMed:12643545, ECO:0000269|PubMed:17081065, ECO:0000269|PubMed:22314232}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P27824
Gene Ontology
(Biological Process)
Complete annatation
aging [GO:0007568];
antigen processing and presentation of exogenous peptide antigen via MHC class II [GO:0019886];
antigen processing and presentation of peptide antigen via MHC class I [GO:0002474];
chaperone-mediated protein folding [GO:0061077];
clathrin-dependent endocytosis [GO:0072583];
protein folding [GO:0006457];
protein folding in endoplasmic reticulum [GO:0034975];
protein secretion [GO:0009306];
synaptic vesicle endocytosis [GO:0048488]
Gene Ontology
(Molecular Function)
Complete annatation
calcium ion binding [GO:0005509];
carbohydrate binding [GO:0030246];
poly(A RNA binding [GO:0044822]
Gene Ontology
(Cellular Component)
Complete annatation
axon [GO:0030424];
cytosol [GO:0005829];
dendrite cytoplasm [GO:0032839];
dendritic spine [GO:0043197];
endoplasmic reticulum [GO:0005783];
endoplasmic reticulum lumen [GO:0005788];
endoplasmic reticulum membrane [GO:0005789];
ER-mitochondrion membrane contact site [GO:0044233];
extracellular exosome [GO:0070062];
integral component of lumenal side of endoplasmic reticulum membrane [GO:0071556];
melanosome [GO:0042470];
membrane [GO:0016020];
myelin sheath [GO:0043209];
neuronal cell body [GO:0043025];
protein complex [GO:0043234];
ribosome [GO:0005840];
rough endoplasmic reticulum [GO:0005791];
smooth endoplasmic reticulum [GO:0005790]
Protein-protein interaction107271
Phylogenetic treeP27824
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.4469636198347350.1722274418891720.271613686737846
AZA vs. DISU0.02207893247917790.9303066848955740.995042856396371
AZA vs. IL70.008831460149083980.963208829231070.999311006273513
AZA vs. SAHA-0.2334149559452240.3375591194096940.709674500136921
DISU vs. CD3-0.4368899731452690.2290879089872120.353059108483103
DISU vs. IL7-0.02229327404974530.9293605000664190.986985597323202
DISU vs. SAHA-0.2548056959830130.3810645947816080.747956662720956
DMSO vs. AZA-0.06961650333105960.6761551915619171
DMSO vs. CD3-0.5277085929527010.09920435346028960.169738795164421
DMSO vs. DISU-0.09364578561314290.7005182159547420.958803444206224
DMSO vs. IL70.08571060577271690.6321742855287780.920378169311359
DMSO vs. SAHA-0.1712653064773310.4663871612227310.792443844769281
HIV vs. Mock in Activation-0.01100945989579680.985897759574750.999983755607037
HIV vs. Mock in Latency-0.01559909075848490.9244333131673390.999834320637052
IL7 vs. CD3-0.4297333580642130.1809134382224450.299213298435812
SAHA vs. CD3-0.705562573167590.04664156983038230.0935562967315135
SAHA vs. IL7-0.2462694982260770.3108418508121370.559879323592084
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
-0.3733 0.02836

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0218503 0.91743
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.001 0.968 1.054 1.107 0.927
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00025 Antihemophilic Factor (Recombinant) approved, investigational unknown chaperone
DB00031 Tenecteplase approved unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp160; precursor binds 20202930
Envelope surface glycoprotein gp120 complexes with 23125841
Nef interacts with 25170080
Gag-Pol complexes with 23125841
Envelope surface glycoprotein gp160; precursor interacts with 22190034
Pr55(Gag) complexes with 23125841
Tat upregulates 23166591
Envelope surface glycoprotein gp120 associates with 10873786
Nef complexes with 23125841

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)
hsa04145 Phagosome - Homo sapiens (human)
hsa04612 Antigen processing and presentation - Homo sapiens (human)
hsa04918 Thyroid hormone synthesis - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)