Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000449
UniProt IDQ8WVQ1
Primary gene name(s)CANT1
Synonym gene name(s)SHAPY
Protein nameSoluble calcium-activated nucleotidase 1
Protein functionCalcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > UTP > GTP. Has very low activity towards ADP and even lower activity towards ATP. Does not hydrolyze AMP and GMP, PubMed:12234496, PubMed:15248776, PubMed:15006348, PubMed:16835225. Involved in proteoglycan synthesis, PubMed:22539336. {ECO:0000269|PubMed:12234496, ECO:0000269|PubMed:15006348, ECO:0000269|PubMed:15248776, ECO:0000269|PubMed:16835225, ECO:0000269|PubMed:22539336}.
Subcellular locationEndoplasmic reticulum membrane {ECO:0000269|PubMed:12234496};
Single-pass type II membrane protein {ECO:0000269|PubMed:12234496}. Golgi apparatus, Golgi stack membrane {ECO:0000269|PubMed:12234496};
Single-pass type II membrane protein {ECO:0000269|PubMed:12234496}. Cell membrane {ECO:0000269|PubMed:16835225}. Note=Processed form: Secreted.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8WVQ1
Gene Ontology
(Biological Process)
Complete annatation
positive regulation of I-kappaB kinase/NF-kappaB signaling [GO:0043123];
proteoglycan biosynthetic process [GO:0030166]
Gene Ontology
(Molecular Function)
Complete annatation
calcium ion binding [GO:0005509];
guanosine-diphosphatase activity [GO:0004382];
signal transducer activity [GO:0004871];
uridine-diphosphatase activity [GO:0045134]
Gene Ontology
(Cellular Component)
Complete annatation
endoplasmic reticulum membrane [GO:0005789];
extracellular exosome [GO:0070062];
Golgi cisterna membrane [GO:0032580];
integral component of membrane [GO:0016021]
Protein-protein interaction125875
Phylogenetic treeQ8WVQ1
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.3982195622253380.2261838520489860.335546516374653
AZA vs. DISU0.06843121200897540.7874438534025890.980024090198827
AZA vs. IL7-0.04441659043006840.8186648379328520.999311006273513
AZA vs. SAHA-0.2734038302674820.2649964214674050.637293563688522
DISU vs. CD3-0.3410754547104830.3497728442175170.48349159594084
DISU vs. IL7-0.1227212354907130.6271278610652080.887560842643647
DISU vs. SAHA-0.3398379972193280.2450901696778040.623418387715473
DMSO vs. AZA-0.06268803426399870.7111061361714931
DMSO vs. CD3-0.4715896685989680.1429716674447230.228284914685188
DMSO vs. DISU-0.1329918782838520.5868873672396470.934701463289767
DMSO vs. IL70.02568464172136950.8872831531105430.975874676784317
DMSO vs. SAHA-0.2163254247365940.361237747227780.709384598439679
HIV vs. Mock in Activation0.2367309664055020.7041366888710120.999983755607037
HIV vs. Mock in Latency0.05110065975485940.7589507599765320.999834320637052
IL7 vs. CD3-0.4371017758122930.1774843666263090.294858999858997
SAHA vs. CD3-0.6951781885434440.0510999747505940.100809317079239
SAHA vs. IL7-0.2310337517566280.3452557039415260.594650877652487
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.241791 0.076331
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.962 1.11 1.181 1.154 1.187
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB03486 Phosphomethylphosphonic Acid Guanosyl Ester experimental unknown unknown
DB03754 Tris approved unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1S18 X-ray 1.7Å A/B=71-401.
1S1D X-ray 1.6Å A/B=71-401.
2H2N X-ray 2.3Å A/B=69-401.
2H2U X-ray 2.4Å A/B=69-401.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
HIV-1 virus replication enhanced by expression of human gene 18854154

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00230 Purine metabolism - Homo sapiens (human)
hsa00240 Pyrimidine metabolism - Homo sapiens (human)