Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000444
UniProt IDQ13555
Primary gene name(s)CAMK2G
Synonym gene name(s)CAMK, CAMK-II, CAMKG
Protein nameCalcium/calmodulin-dependent protein kinase type II subunit gamma
Protein functionCalcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+/calmodulin-binding and autophosphorylation, and is involved in sarcoplsamic reticulum Ca(2+ transport in skeletal muscle and may function in dendritic spine and synapse formation and neuronal plasticity. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum, SR Ca(2+ transport and in fast-twitch muscle participates in the control of Ca(2+ release from the SR through phosphorylation of the ryanodine receptor-coupling factor triadin. In neurons, may participate in the promotion of dendritic spine and synapse formation and maintenance of synaptic plasticity which enables long-term potentiation, LTP and hippocampus-dependent learning. {ECO:0000269|PubMed:16690701}.
Subcellular locationSarcoplasmic reticulum membrane {ECO:0000305};
Peripheral membrane protein {ECO:0000305};
Cytoplasmic side {ECO:0000305}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q13555
Gene Ontology
(Biological Process)
Complete annatation
calcium ion transport [GO:0006816];
cell differentiation [GO:0030154];
G1/S transition of mitotic cell cycle [GO:0000082];
insulin secretion [GO:0030073];
interferon-gamma-mediated signaling pathway [GO:0060333];
MAPK cascade [GO:0000165];
nervous system development [GO:0007399];
protein autophosphorylation [GO:0046777];
protein oligomerization [GO:0051259];
regulation of calcium ion transport [GO:0051924];
regulation of cellular response to heat [GO:1900034];
regulation of relaxation of cardiac muscle [GO:1901897];
regulation of skeletal muscle adaptation [GO:0014733]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
calcium-dependent protein serine/threonine phosphatase activity [GO:0004723];
calmodulin binding [GO:0005516];
calmodulin-dependent protein kinase activity [GO:0004683];
protein homodimerization activity [GO:0042803];
protein serine/threonine kinase activity [GO:0004674];
Ras guanyl-nucleotide exchange factor activity [GO:0005088]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
endocytic vesicle membrane [GO:0030666];
membrane [GO:0016020];
neuronal postsynaptic density [GO:0097481];
nucleoplasm [GO:0005654];
plasma membrane [GO:0005886];
sarcoplasmic reticulum membrane [GO:0033017]
Protein-protein interaction107268
Phylogenetic treeQ13555
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-2.320735584398521.74223884208402e-081.95670818389645e-07
AZA vs. DISU0.1157280469062160.6478126969815470.960057478314726
AZA vs. IL7-0.1961915367517720.308083558827910.999311006273513
AZA vs. SAHA-0.07184504013409080.7685637758021470.938615438421904
DISU vs. CD32.423651635294258.13625489204384e-091.29373318821381e-07
DISU vs. IL7-0.3212029897944290.2031064424263340.580551691235682
DISU vs. SAHA-0.1854348987199930.5266605230683470.83957138019561
DMSO vs. AZA0.03723352253816880.8242617681865471
DMSO vs. CD32.344939373558151.2032999263667e-081.26590177309457e-07
DMSO vs. DISU-0.08069187530452830.741242421834450.968318302887802
DMSO vs. IL7-0.2259940043847950.2094041958635780.718427003332597
DMSO vs. SAHA-0.1146707690014370.6269404761331780.878206145162481
HIV vs. Mock in Activation0.3003151275446970.7353885235990610.999983755607037
HIV vs. Mock in Latency-0.07018012630860440.6706773531903860.999834320637052
IL7 vs. CD32.131009171676391.97861992345949e-072.26796487500417e-06
SAHA vs. CD32.224557817388981.74719539169921e-071.71420797870489e-06
SAHA vs. IL70.1210002574793970.6199387129687890.810069015195174
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.50057 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.038 1.059 1.201 1.212 1.015
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB08699 1-tert-butyl-3-(3-methylbenzyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine experimental unknown unknown
DB06616 Bosutinib approved unknown inhibitor

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2UX0 X-ray 2.4Å A/B/C/D/E/F=389-556.
2V7O X-ray 2.2Å A=5-315.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat activates 24198379
Tat upregulates 25613138

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04012 ErbB signaling pathway - Homo sapiens (human)
hsa04020 Calcium signaling pathway - Homo sapiens (human)
hsa04024 cAMP signaling pathway - Homo sapiens (human)
hsa04066 HIF-1 signaling pathway - Homo sapiens (human)
hsa04114 Oocyte meiosis - Homo sapiens (human)
hsa04261 Adrenergic signaling in cardiomyocytes - Homo sapiens (human)
hsa04310 Wnt signaling pathway - Homo sapiens (human)
hsa04360 Axon guidance - Homo sapiens (human)
hsa04713 Circadian entrainment - Homo sapiens (human)
hsa04720 Long-term potentiation - Homo sapiens (human)
hsa04722 Neurotrophin signaling pathway - Homo sapiens (human)
hsa04725 Cholinergic synapse - Homo sapiens (human)
hsa04728 Dopaminergic synapse - Homo sapiens (human)
hsa04740 Olfactory transduction - Homo sapiens (human)
hsa04750 Inflammatory mediator regulation of TRP channels - Homo sapiens (human)
hsa04911 Insulin secretion - Homo sapiens (human)
hsa04912 GnRH signaling pathway - Homo sapiens (human)
hsa04916 Melanogenesis - Homo sapiens (human)
hsa04921 Oxytocin signaling pathway - Homo sapiens (human)
hsa04922 Glucagon signaling pathway - Homo sapiens (human)
hsa04925 Aldosterone synthesis and secretion - Homo sapiens (human)
hsa04971 Gastric acid secretion - Homo sapiens (human)
hsa05031 Amphetamine addiction - Homo sapiens (human)
hsa05152 Tuberculosis - Homo sapiens (human)
hsa05205 Proteoglycans in cancer - Homo sapiens (human)
hsa05214 Glioma - Homo sapiens (human)