Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000442
UniProt IDQ8IU85
Primary gene name(s)CAMK1D
Synonym gene name(s)CAMKID
Protein nameCalcium/calmodulin-dependent protein kinase type 1D
Protein functionCalcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, activates CREB-dependent gene transcription, regulates calcium-mediated granulocyte function and respiratory burst and promotes basal dendritic growth of hippocampal neurons. In neutrophil cells, required for cytokine-induced proliferative responses and activation of the respiratory burst. Activates the transcription factor CREB1 in hippocampal neuron nuclei. May play a role in apoptosis of erythroleukemia cells. In vitro, phosphorylates transcription factor CREM isoform Beta. {ECO:0000269|PubMed:11050006, ECO:0000269|PubMed:15840691, ECO:0000269|PubMed:16324104, ECO:0000269|PubMed:17056143}.
Subcellular locationCytoplasm {ECO:0000305}. Nucleus {ECO:0000305}. Note=Predominantly cytoplasmic. Nuclear localization increases upon activation by KCl treatment in hippocampal neurons.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8IU85
Gene Ontology
(Biological Process)
Complete annatation
inflammatory response [GO:0006954];
negative regulation of apoptotic process [GO:0043066];
positive regulation of apoptotic process [GO:0043065];
positive regulation of CREB transcription factor activity [GO:0032793];
positive regulation of neuron projection development [GO:0010976];
positive regulation of neutrophil chemotaxis [GO:0090023];
positive regulation of phagocytosis [GO:0050766];
positive regulation of respiratory burst [GO:0060267];
regulation of dendrite development [GO:0050773];
regulation of granulocyte chemotaxis [GO:0071622]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
calmodulin-dependent protein kinase activity [GO:0004683]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
nucleus [GO:0005634]
Protein-protein interaction121382
Phylogenetic treeQ8IU85
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      Yes - Two siRNA pools inhibit HIV replication and inhibition of Tat-mediated transactivation of the HIV LTR is also observed
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.977926936615580.007372522032105590.0201245354463677
AZA vs. DISU0.2700327071389250.2928676461360180.858710275220237
AZA vs. IL7-0.2862166893028190.1439999111216910.908692496481083
AZA vs. SAHA-0.7481407073414820.05341125672187840.281412542406709
DISU vs. CD32.236082036953690.00292372612743030.0103407432296772
DISU vs. IL7-0.5668554600579770.04797035285674830.289293346017902
DISU vs. SAHA-1.015703650394920.02606672765772210.189783407019097
DMSO vs. AZA0.04411411707223840.7964155728819651
DMSO vs. CD32.013869873218890.007855051277253830.0202388143478415
DMSO vs. DISU-0.2271461079925330.3888166391772630.86028854108019
DMSO vs. IL7-0.3236587144673420.1392917111852660.638437989090265
DMSO vs. SAHA-0.7988409903356190.04657385674192260.242123006194383
HIV vs. Mock in Activation0.4100136624871190.7784065902419410.999983755607037
HIV vs. Mock in Latency0.3468871782126360.03846211152163340.762865617206712
IL7 vs. CD31.696107346680880.02695699340887040.0666539963339736
SAHA vs. CD31.205604419070040.1783147933907930.276432870733387
SAHA vs. IL7-0.4650860315943130.257403770829540.502213880595973
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
0.3534 0.02772

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.433217 0.0130969
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.986 1.018 1.241 1.191 1.251
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB08454 N-(5-METHYL-1H-PYRAZOL-3-YL)-2-PHENYLQUINAZOLIN-4-AMINE experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2JC6 X-ray 2.3Å A/C=1-333.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
HIV-1 virus replication enhanced by expression of human gene 18976975

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04921 Oxytocin signaling pathway - Homo sapiens (human)
hsa04925 Aldosterone synthesis and secretion - Homo sapiens (human)