Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000436
UniProt IDP27708
Primary gene name(s)CAD
Synonym gene name(s)unknown
Protein nameCAD protein [Includes: Glutamine-dependent carbamoyl-phosphate synthase
Protein functionThis protein is a ""fusion"" protein encoding four enzymatic activities of the pyrimidine pathway, GATase, CPSase, ATCase and DHOase. {ECO:0000269|PubMed:24332717}."
Subcellular locationCytoplasm {ECO:0000269|PubMed:15890648}. Nucleus {ECO:0000269|PubMed:15890648}. Note=Cytosolic and unphosphorylated in resting cells, translocates to the nucleus in response to EGF stimulation, nuclear import promotes optimal cell growth.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P27708
Gene Ontology
(Biological Process)
Complete annatation
'de novo' pyrimidine nucleobase biosynthetic process [GO:0006207];
'de novo' UMP biosynthetic process [GO:0044205];
animal organ regeneration [GO:0031100];
arginine biosynthetic process [GO:0006526];
cellular response to drug [GO:0035690];
cellular response to epidermal growth factor stimulus [GO:0071364];
citrulline biosynthetic process [GO:0019240];
drug metabolic process [GO:0017144];
female pregnancy [GO:0007565];
glutamine metabolic process [GO:0006541];
heart development [GO:0007507];
lactation [GO:0007595];
liver development [GO:0001889];
peptidyl-threonine phosphorylation [GO:0018107];
protein autophosphorylation [GO:0046777];
pyrimidine nucleoside biosynthetic process [GO:0046134];
response to amine [GO:0014075];
response to caffeine [GO:0031000];
response to cortisol [GO:0051414];
response to insulin [GO:0032868];
response to starvation [GO:0042594];
response to testosterone [GO:0033574];
urea cycle [GO:0000050];
UTP biosynthetic process [GO:0006228]
Gene Ontology
(Molecular Function)
Complete annatation
aspartate binding [GO:0070335];
aspartate carbamoyltransferase activity [GO:0004070];
ATP binding [GO:0005524];
carbamoyl-phosphate synthase, ammonia activity [GO:0004087];
carbamoyl-phosphate synthase, glutamine-hydrolyzing activity [GO:0004088];
dihydroorotase activity [GO:0004151];
enzyme binding [GO:0019899];
identical protein binding [GO:0042802];
protein kinase activity [GO:0004672];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
cell projection [GO:0042995];
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
membrane [GO:0016020];
neuronal cell body [GO:0043025];
nuclear matrix [GO:0016363];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
protein complex [GO:0043234];
terminal bouton [GO:0043195]
Protein-protein interaction107243
Phylogenetic treeP27708
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.971419177695510.003243639157925690.0100737954643017
AZA vs. DISU-0.5390831318026230.09047478767089710.622436825864682
AZA vs. IL70.3322504316492520.1222042302364420.887275704409388
AZA vs. SAHA-0.6040859036927540.01412094470968360.122332827904466
DISU vs. CD3-1.52306239431524.03197937469679e-050.000255584764904035
DISU vs. IL70.8622432454962370.004121642289895310.0665618148948801
DISU vs. SAHA-0.06299167549733470.8311586487905310.956286128400802
DMSO vs. AZA0.1098161546311510.5864041440072231
DMSO vs. CD3-0.8756513890123110.00647919478808490.0171275363339049
DMSO vs. DISU0.646406859096210.02655460725096160.33320393944379
DMSO vs. IL70.2301801374369750.2001065116877350.711128162115181
DMSO vs. SAHA-0.7190255455503210.002462983026310870.0338501948243122
HIV vs. Mock in Activation0.1424580414946950.8186729796883810.999983755607037
HIV vs. Mock in Latency-0.2378055776937950.1510888175746040.999834320637052
IL7 vs. CD3-0.6324617776256340.04947413345217930.108845415968731
SAHA vs. CD3-1.599361933581761.02314576594376e-056.41648218592277e-05
SAHA vs. IL7-0.9394150322540570.0001347033710438210.0024702172933267
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change -0.961758385
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.394098 0.00241278
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.964 0.975 0.927 0.87 0.953
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB03459 Sparfosic acid experimental unknown unknown
DB00128 L-Aspartic Acid approved, nutraceutical unknown unknown
DB00130 L-Glutamine approved, investigational, nutraceutical unknown substrate

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4BY3 X-ray 1.7Å A=1456-1846.
4C6B X-ray 1.6Å A=1456-1846.
4C6C X-ray 1.4Å A=1456-1846.
4C6D X-ray 1.3Å A=1456-1846.
4C6E X-ray 1.2Å A=1456-1846.
4C6F X-ray 1.2Å A=1456-1846.
4C6I X-ray 1.3Å A=1456-1846.
4C6J X-ray 1.3Å A=1456-1846.
4C6K X-ray 1.4Å A=1456-1846.
4C6L X-ray 1.5Å A=1456-1846.
4C6M X-ray 1.6Å A=1456-1846.
4C6N X-ray 1.9Å A=1456-1846.
4C6O X-ray 1.6Å A=1456-1846.
4C6P X-ray 1.5Å A=1456-1846.
4C6Q X-ray 1.6Å A=1456-1846.
5G1N X-ray 2.1Å A/B/C/D/E/F=1915-2225.
5G1O X-ray 2.1Å A/B/C/D/E/F=1915-2225.
5G1P X-ray 3.1Å A/B/C/D/E/F=1915-2225.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 complexes with 23125841
Gag-Pol complexes with 23125841
Pr55(Gag) complexes with 23125841
HIV-1 virus replication enhanced by expression of human gene 18854154
Nef complexes with 23125841

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00240 Pyrimidine metabolism - Homo sapiens (human)
hsa00250 Alanine# aspartate and glutamate metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
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