Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000275
UniProt IDO43684
Primary gene name(s)BUB3
Synonym gene name(s)unknown
Protein nameMitotic checkpoint protein BUB3
Protein functionHas a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule, K-MT attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome, APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. {ECO:0000269|PubMed:10198256, ECO:0000269|PubMed:15525512, ECO:0000269|PubMed:18199686}.
Subcellular locationNucleus. Chromosome, centromere, kinetochore {ECO:0000250}. Note=Starts to localize at kinetochores in prometaphase I, Pro-MI stage and maintains the localization until the metaphase I-anaphase I, MI-AI transition. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O43684
Gene Ontology
(Biological Process)
Complete annatation
anaphase-promoting complex-dependent catabolic process [GO:0031145];
attachment of spindle microtubules to kinetochore [GO:0008608];
cell division [GO:0051301];
meiotic cell cycle [GO:0051321];
mitotic sister chromatid segregation [GO:0000070];
mitotic spindle assembly checkpoint [GO:0007094];
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [GO:0051436];
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition [GO:0051437];
proteasome-mediated ubiquitin-dependent protein catabolic process [GO:0043161];
protein ubiquitination involved in ubiquitin-dependent protein catabolic process [GO:0042787];
sister chromatid cohesion [GO:0007062];
spindle assembly checkpoint [GO:0071173]
Gene Ontology
(Molecular Function)
Complete annatation
ubiquitin binding [GO:0043130]
Gene Ontology
(Cellular Component)
Complete annatation
bub1-bub3 complex [GO:1990298];
condensed chromosome kinetochore [GO:0000777];
cytosol [GO:0005829];
kinetochore [GO:0000776];
mitotic checkpoint complex [GO:0033597];
nucleoplasm [GO:0005654]
Protein-protein interaction114621
Phylogenetic treeO43684
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.3352046950875590.3070661578761650.42592500750134
AZA vs. DISU0.08689148439332370.7308058916690250.974560663114398
AZA vs. IL70.02967874910519220.8770669115400320.999311006273513
AZA vs. SAHA-0.1510993786768660.535045998705890.840757620724223
DISU vs. CD3-0.2611121628983670.4713900645663850.599488216761468
DISU vs. IL7-0.06648663952878880.7914681714358420.95268146633626
DISU vs. SAHA-0.2362462200084340.4170963083065930.771430478236783
DMSO vs. AZA-0.02122369458285790.8989392341318991
DMSO vs. CD3-0.3673894513252430.2518777663460930.357907805131475
DMSO vs. DISU-0.1097449772322340.6523895341433550.949713766615536
DMSO vs. IL70.05802683087320910.7464185104962710.950269764295326
DMSO vs. SAHA-0.1360915631547260.5631452510238980.845671789376362
HIV vs. Mock in Activation0.002924524620767710.9962447822700030.999983755607037
HIV vs. Mock in Latency0.1436990742403280.3831443827323910.999834320637052
IL7 vs. CD3-0.2985218627076580.353104327210250.49035761231252
SAHA vs. CD3-0.5102863197339110.1503058432218820.241324067453935
SAHA vs. IL7-0.1836040588202750.4503268548039530.688950269898994
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0432596 0.821882
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.989 1.049 0.982 0.921 0.922
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04110 Cell cycle - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)