Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000261
UniProt IDQ8TDC3
Primary gene name(s)BRSK1
Synonym gene name(s)KIAA1811, SAD1, SADB
Protein nameSerine/threonine-protein kinase BRSK1
Protein functionSerine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma-tubulin, TUBG1 and TUBG2 at 'Ser-131', leading to translocation of gamma-tubulin and its associated proteins to the centrosome. Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C. {ECO:0000269|PubMed:14976552, ECO:0000269|PubMed:15150265, ECO:0000269|PubMed:20026642, ECO:0000269|PubMed:21985311}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:15150265}. Nucleus {ECO:0000269|PubMed:15150265}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250}. Cell junction, synapse {ECO:0000250}. Note=Localizes to synaptic vesicles in neurons, By similarity. Nuclear in the absence of DNA damage. Translocated to the nucleus in response to UV- or MMS-induced DNA damage. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8TDC3
Gene Ontology
(Biological Process)
Complete annatation
axonogenesis [GO:0007409];
cellular response to DNA damage stimulus [GO:0006974];
centrosome duplication [GO:0051298];
establishment of cell polarity [GO:0030010];
G2 DNA damage checkpoint [GO:0031572];
intracellular signal transduction [GO:0035556];
neuron differentiation [GO:0030182];
neurotransmitter secretion [GO:0007269];
protein phosphorylation [GO:0006468];
response to UV [GO:0009411]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
gamma-tubulin binding [GO:0043015];
magnesium ion binding [GO:0000287];
protein serine/threonine kinase activity [GO:0004674];
tau-protein kinase activity [GO:0050321]
Gene Ontology
(Cellular Component)
Complete annatation
cell junction [GO:0030054];
centrosome [GO:0005813];
cytoplasm [GO:0005737];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
synaptic vesicle [GO:0008021]
Protein-protein interaction124084
Phylogenetic treeQ8TDC3
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.407124704616830.0004163674068454750.00169105081001751
AZA vs. DISU-0.07936445006293550.7929328991431430.981325057307903
AZA vs. IL7-0.0598246200029350.8347341993571160.999311006273513
AZA vs. SAHA-0.8540935683475090.09913272274043780.398274696852175
DISU vs. CD31.314804619342420.001591723963418330.00616327948229334
DISU vs. IL70.008329474860671150.9780250107003980.997213340844928
DISU vs. SAHA-0.7702909659985140.1495938630796210.496963050836263
DMSO vs. AZA0.06440455333598910.8138053882472411
DMSO vs. CD31.465629916892040.0002443447688559310.000994879444003945
DMSO vs. DISU0.1430660382932010.6247254124360230.94481879965491
DMSO vs. IL7-0.1176647594218580.6695980375786840.93213494903488
DMSO vs. SAHA-0.9236391025866960.07173420355268170.312910617002161
HIV vs. Mock in Activation0.7223067332744090.2858857286266310.999983755607037
HIV vs. Mock in Latency-0.08598473407965870.6864936032222360.999834320637052
IL7 vs. CD31.350789874325090.0006335490710499550.00287375418234606
SAHA vs. CD30.5343879769493120.3469711079310820.463107297168003
SAHA vs. IL7-0.7941058794095970.1222456455701550.323968154241301
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.209606 0.704711
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found