Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000239
UniProt IDQ9NYF8
Primary gene name(s)BCLAF1
Synonym gene name(s)BTF, KIAA0164
Protein nameBcl-2-associated transcription factor 1
Protein functionDeath-promoting transcriptional repressor. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. {ECO:0000269|PubMed:18794151}.
Subcellular locationCytoplasm. Nucleus. Nucleus speckle {ECO:0000269|PubMed:24100041}. Nucleus, nucleoplasm {ECO:0000269|PubMed:24100041}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9NYF8
Gene Ontology
(Biological Process)
Complete annatation
apoptotic process [GO:0006915];
negative regulation of transcription, DNA-templated [GO:0045892];
positive regulation of apoptotic process [GO:0043065];
positive regulation of DNA-templated transcription, initiation [GO:2000144];
positive regulation of intrinsic apoptotic signaling pathway [GO:2001244];
positive regulation of response to DNA damage stimulus [GO:2001022];
regulation of DNA-templated transcription in response to stress [GO:0043620];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
DNA binding [GO:0003677];
poly(A RNA binding [GO:0044822]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
nuclear speck [GO:0016607];
nucleolus [GO:0005730];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction115118
Phylogenetic treeQ9NYF8
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      Yes - Two siRNA pools inhibit HIV replication and inhibition of Tat-mediated transactivation of the HIV LTR is not observed
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.6566284644425990.04540311340585450.0921761483368856
AZA vs. DISU0.313433918487880.2182853195045880.807266074519541
AZA vs. IL70.08020858558817120.6754513527634990.999311006273513
AZA vs. SAHA-0.6580935022020730.007384371898338070.0799081277785701
DISU vs. CD3-0.3560762164991230.3309777734151260.464122227710652
DISU vs. IL7-0.2417200299669460.3404583765320590.721316413158482
DISU vs. SAHA-0.9713681367852140.0009349019394834230.0198729296614311
DMSO vs. AZA-0.03351298587376020.8407456083295581
DMSO vs. CD3-0.7021566121094310.02856012729529570.0603109500565783
DMSO vs. DISU-0.3489340058797050.1554146898585310.657838022105253
DMSO vs. IL70.1210774994531950.4991860142600320.885914818891506
DMSO vs. SAHA-0.6322247052341530.007712006907389710.0758666162540929
HIV vs. Mock in Activation-0.05285606862756380.9323404107232810.999983755607037
HIV vs. Mock in Latency0.01928999874884450.9065277863368370.999834320637052
IL7 vs. CD3-0.5671825884937840.07780853366259290.156166182143311
SAHA vs. CD3-1.340688265800340.0002055336124398010.000902503362315793
SAHA vs. IL7-0.7431665675109940.002467171340732490.02298525388764
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0963444 0.549073
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.051 0.994 0.942 0.827 0.941
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category