Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000232
UniProt IDQ9H6U6
Primary gene name(s)BCAS3
Synonym gene name(s)unknown
Protein nameBreast carcinoma-amplified sequence 3
Protein functionPlays a role in angiogenesis. Participates in the regulation of cell polarity and directional endothelial cell migration by mediating both the activation and recruitment of CDC42 and the reorganization of the actin cytoskeleton at the cell leading edge. Promotes filipodia formation, By similarity. Functions synergistically with PELP1 as a transcriptional coactivator of estrogen receptor-responsive genes. Stimulates histone acetyltransferase activity. Binds to chromatin. {ECO:0000250|UniProtKB:Q8CCN5, ECO:0000269|PubMed:17505058}.
Subcellular locationNucleus {ECO:0000269|PubMed:16617102, ECO:0000269|PubMed:17505058}. Cytoplasm {ECO:0000269|PubMed:16617102, ECO:0000269|PubMed:17505058, ECO:0000269|PubMed:18030336}. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q8CCN5}. Note=Localizes in the cytoplasm in stationary cells. Translocates from the cytoplasm to the leading edge in motile cells. Colocalizes with microtubules and intermediate filaments in both stationary and motile cells, By similarity. Associates with chromatin. Recruited to estrogen receptor-induced promoters in a PELP1-dependent manner. {ECO:0000250|UniProtKB:Q8CCN5, ECO:0000269|PubMed:17505058}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9H6U6
Gene Ontology
(Biological Process)
Complete annatation
activation of GTPase activity [GO:0090630];
angiogenesis [GO:0001525];
cellular response to estrogen stimulus [GO:0071391];
Golgi organization [GO:0007030];
microtubule organizing center organization [GO:0031023];
negative regulation of focal adhesion assembly [GO:0051895];
negative regulation of GTPase activity [GO:0034260];
positive regulation of actin cytoskeleton reorganization [GO:2000251];
positive regulation of catalytic activity [GO:0043085];
positive regulation of endothelial cell migration [GO:0010595];
positive regulation of filopodium assembly [GO:0051491];
positive regulation of GTPase activity [GO:0043547];
positive regulation of intracellular protein transport [GO:0090316];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
regulation of establishment of cell polarity [GO:2000114];
response to starvation [GO:0042594];
transcription, DNA-templated [GO:0006351];
tube formation [GO:0035148];
vesicle-mediated transport [GO:0016192]
Gene Ontology
(Molecular Function)
Complete annatation
acetyltransferase activator activity [GO:0010698];
beta-tubulin binding [GO:0048487];
chromatin binding [GO:0003682];
histone acetyltransferase binding [GO:0035035];
histone binding [GO:0042393];
nuclear hormone receptor binding [GO:0035257];
transcription factor binding [GO:0008134]
Gene Ontology
(Cellular Component)
Complete annatation
cell leading edge [GO:0031252];
cell periphery [GO:0071944];
cytoplasm [GO:0005737];
cytoplasmic microtubule [GO:0005881];
intermediate filament cytoskeleton [GO:0045111];
nucleolus [GO:0005730];
nucleus [GO:0005634];
transcriptionally active chromatin [GO:0035327]
Protein-protein interaction120182
Phylogenetic treeQ9H6U6
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-2.650039533414521.98669279797281e-050.000114604204800403
AZA vs. DISU0.03104814685244260.9043360210749250.993937521279788
AZA vs. IL7-0.293339423568810.1413500252326770.90607959737543
AZA vs. SAHA-0.5817956804200330.1287910668502960.457117958049028
DISU vs. CD32.67080794286293.28114959744497e-050.000213098013924539
DISU vs. IL7-0.3335180821085040.1943336122563140.568646803166237
DISU vs. SAHA-0.6115040188314090.15298886440070.502877900846882
DMSO vs. AZA0.03802217960677360.8279667461539811
DMSO vs. CD32.676239346672121.83935820666781e-059.94970606228026e-05
DMSO vs. DISU0.004564523028281480.985332266294280.997779683715549
DMSO vs. IL7-0.3235092316027270.08296466565802560.539091523618421
DMSO vs. SAHA-0.6259646286160090.1102251677189920.393930164262016
HIV vs. Mock in Activation0.1563830869523920.9033183701789580.999983755607037
HIV vs. Mock in Latency0.07413040979827120.6633499000282650.999834320637052
IL7 vs. CD32.364710649280570.0001478819857179130.000821799748921649
SAHA vs. CD32.047718916721220.003264758504389790.00986959090494565
SAHA vs. IL7-0.2931147774799840.4559238780387410.692941776321433
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -1.08616 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.971 1.002 1.148 1.164 1.127
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found