Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000196
UniProt IDQ9Y2Q0
Primary gene name(s)ATP8A1
Synonym gene name(s)ATPIA
Protein namePhospholipid-transporting ATPase IA
Protein functionCatalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. In vitro, its ATPase activity is selectively and stereospecifically stimulated by phosphatidylserine, PS. The flippase complex ATP8A1:TMEM30A seems to play a role in regulation of cell migration probably involving flippase-mediated translocation of phosphatidylethanolamine, PE at the plasma membrane. Acts as aminophospholipid translocase at the plasma membrane in neuronal cells.
Subcellular locationCytoplasmic vesicle, secretory vesicle, chromaffin granule membrane {ECO:0000250|UniProtKB:P70704};
Multi-pass membrane protein {ECO:0000250|UniProtKB:P70704}. Cytoplasmic granule {ECO:0000269|PubMed:20947505}. Cell membrane {ECO:0000269|PubMed:20947505, ECO:0000269|PubMed:21914794}. Endoplasmic reticulum {ECO:0000269|PubMed:20947505, ECO:0000269|PubMed:21914794}. Golgi apparatus {ECO:0000269|PubMed:20947505, ECO:0000269|PubMed:21914794}. Note=Exit from the endoplasmic reticulum requires the presence of TMEM30A, but not TMEM30B, PubMed:20947505. In the presence of TMEM30A, predominantly located in cytoplasmic punctate structures and localizes to the plasma membrane, PubMed:20947505. Localizes to plasma membranes of red blood cells, By similarity. {ECO:0000250|UniProtKB:P70704, ECO:0000269|PubMed:20947505}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9Y2Q0
Gene Ontology
(Biological Process)
Complete annatation
Golgi vesicle budding [GO:0048194];
ion transmembrane transport [GO:0034220];
learning [GO:0007612];
phospholipid translocation [GO:0045332];
positive regulation of cell migration [GO:0030335];
positive regulation of phospholipid translocation [GO:0061092]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
cation-transporting ATPase activity [GO:0019829];
magnesium ion binding [GO:0000287];
phospholipid-translocating ATPase activity [GO:0004012]
Gene Ontology
(Cellular Component)
Complete annatation
chromaffin granule membrane [GO:0042584];
endoplasmic reticulum [GO:0005783];
extracellular exosome [GO:0070062];
Golgi apparatus [GO:0005794];
Golgi membrane [GO:0000139];
integral component of membrane [GO:0016021];
intracellular membrane-bounded organelle [GO:0043231];
membrane [GO:0016020];
plasma membrane [GO:0005886];
trans-Golgi network [GO:0005802]
Protein-protein interaction115668
Phylogenetic treeQ9Y2Q0
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.9793619025565850.01157122473289250.0295145539303022
AZA vs. DISU-0.4845189297867550.05605274776502940.529024520717537
AZA vs. IL7-0.03405882254166280.8595001885814680.999311006273513
AZA vs. SAHA0.5896623670981910.01593166938309810.132389186483363
DISU vs. CD30.4822738531379880.2117639754552850.333280497355722
DISU vs. IL70.4417093728400480.08014957838516390.375643780107955
DISU vs. SAHA1.074979716582350.0002620296070768860.00778953695257469
DMSO vs. AZA0.03135126273840920.8517232031149261
DMSO vs. CD30.9996265058468940.009512391160558460.023876070115597
DMSO vs. DISU0.5142969662950460.0355466521877850.380032012010567
DMSO vs. IL7-0.05828218858767930.7460338363154550.950269764295326
DMSO vs. SAHA0.5510633648840070.01968795977432140.140159301728293
HIV vs. Mock in Activation-0.1478265302684280.8626795265042720.999983755607037
HIV vs. Mock in Latency0.2512601555981040.1282384712442670.999834320637052
IL7 vs. CD30.9536176295949480.01403513773727430.0393730934513187
SAHA vs. CD31.543706092308047.71424908749818e-050.000381284794190218
SAHA vs. IL70.6193596063174280.0112540016652380.0675867813154575
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander -0.769 4.27E-06 5.67E-05
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.129996 0.475348
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00144 Phosphatidyl serine approved, nutraceutical unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found