Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000195
UniProt IDP35670
Primary gene name(s)ATP7B
Synonym gene name(s)PWD, WC1, WND
Protein nameCopper-transporting ATPase 2
Protein functionInvolved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile. {ECO:0000269|PubMed:18203200, ECO:0000269|PubMed:22240481, ECO:0000269|PubMed:24706876}.
Subcellular locationGolgi apparatus, trans-Golgi network membrane {ECO:0000269|PubMed:17919502, ECO:0000269|PubMed:22240481, ECO:0000269|PubMed:24706876};
Multi-pass membrane protein {ECO:0000255}. Late endosome {ECO:0000269|PubMed:11231950, ECO:0000269|PubMed:15681833}. Note=Predominantly found in the trans-Golgi network, TGN. Localized in the trans-Golgi network under low copper conditions, redistributes to cytoplasmic vesicles when cells are exposed to elevated copper levels, and then recycles back to the trans-Golgi network when copper is removed, PubMed:10942420. Not redistributed to the plasma membrane in response to elevated copper levels. {ECO:0000269|PubMed:10942420, ECO:0000269|PubMed:22240481, ECO:0000269|PubMed:24706876, ECO:0000269|PubMed:9307043}.;
SUBCELLULAR LOCATION: Isoform 1: Golgi apparatus membrane {ECO:0000269|PubMed:9307043};
Multi-pass membrane protein {ECO:0000269|PubMed:9307043}.;
SUBCELLULAR LOCATION: Isoform 2: Cytoplasm {ECO:0000269|PubMed:9307043}.;
SUBCELLULAR LOCATION: WND/140 kDa: Mitochondrion {ECO:0000269|PubMed:9600907}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P35670
Gene Ontology
(Biological Process)
Complete annatation
cellular copper ion homeostasis [GO:0006878];
cellular zinc ion homeostasis [GO:0006882];
copper ion import [GO:0015677];
copper ion transport [GO:0006825];
intracellular copper ion transport [GO:0015680];
ion transmembrane transport [GO:0034220];
lactation [GO:0007595];
response to copper ion [GO:0046688];
sequestering of calcium ion [GO:0051208]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
copper-exporting ATPase activity [GO:0004008];
copper ion binding [GO:0005507];
copper-transporting ATPase activity [GO:0043682];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
bicellular tight junction [GO:0005923];
Golgi membrane [GO:0000139];
integral component of plasma membrane [GO:0005887];
late endosome [GO:0005770];
membrane [GO:0016020];
mitochondrion [GO:0005739];
trans-Golgi network membrane [GO:0032588]
Protein-protein interaction107022
Phylogenetic treeP35670
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.6191817402591010.2474022366556630.35954551257103
AZA vs. DISUunknownunknownunknown
AZA vs. IL7unknownunknownunknown
AZA vs. SAHA1.767720443412641.33078962805122e-060.000120489398452768
DISU vs. CD3-0.4824378658967870.279547566926520.408918003284848
DISU vs. IL7unknownunknownunknown
DISU vs. SAHA1.630699797949051.08068182125276e-060.000103738571516563
DMSO vs. AZAunknownunknownunknown
DMSO vs. CD3-0.5713466030174880.2723864199035290.380854769781546
DMSO vs. DISUunknownunknownunknown
DMSO vs. IL7unknownunknownunknown
DMSO vs. SAHA1.700618243526363.94375379264567e-060.000259547220126475
HIV vs. Mock in Activation0.1560237767694780.8734511515798730.999983755607037
HIV vs. Mock in Latencyunknownunknownunknown
IL7 vs. CD3-0.4331215285070980.3967379899986760.533597905170879
SAHA vs. CD31.134937696274160.0146646711345070.0354274757930459
SAHA vs. IL71.601445355962151.19946279697469e-065.31785358870486e-05
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.157122 0.707675
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00515 Cisplatin approved unknown substrate
DB00958 Carboplatin approved unknown substrate
DB00526 Oxaliplatin approved, investigational unknown substrate

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2ARF NMR - A=1032-1196.
2EW9 NMR - A=486-633.
2KOY NMR - A=1036-1196.
2LQB NMR - A=141-212.
2N7Y NMR - A=56-127.
2ROP NMR - A=238-439.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa01524 Platinum drug resistance - Homo sapiens (human)