Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000194
UniProt IDQ04656
Primary gene name(s)ATP7A
Synonym gene name(s)MC1, MNK
Protein nameCopper-transporting ATPase 1
Protein functionMay supply copper to copper-requiring proteins within the secretory pathway, when localized in the trans-Golgi network. Under conditions of elevated extracellular copper, it relocalized to the plasma membrane where it functions in the efflux of copper from cells.
Subcellular locationGolgi apparatus, trans-Golgi network membrane {ECO:0000269|PubMed:10484781, ECO:0000269|PubMed:9147644, ECO:0000269|PubMed:9467005};
Multi-pass membrane protein {ECO:0000255}. Cell membrane {ECO:0000269|PubMed:10484781, ECO:0000269|PubMed:9147644};
Multi-pass membrane protein {ECO:0000255}. Note=Cycles constitutively between the trans-Golgi network, TGN and the plasma membrane, PubMed:9147644. Predominantly found in the TGN and relocalized to the plasma membrane in response to elevated copper levels. {ECO:0000269|PubMed:9147644}.;
SUBCELLULAR LOCATION: Isoform 3: Cytoplasm, cytosol {ECO:0000305}.;
SUBCELLULAR LOCATION: Isoform 5: Endoplasmic reticulum {ECO:0000269|PubMed:9467005}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q04656
Gene Ontology
(Biological Process)
Complete annatation
blood vessel development [GO:0001568];
blood vessel remodeling [GO:0001974];
cartilage development [GO:0051216];
catecholamine metabolic process [GO:0006584];
cellular copper ion homeostasis [GO:0006878];
central nervous system neuron development [GO:0021954];
cerebellar Purkinje cell differentiation [GO:0021702];
collagen fibril organization [GO:0030199];
copper ion export [GO:0060003];
copper ion import [GO:0015677];
copper ion transport [GO:0006825];
detoxification of copper ion [GO:0010273];
dopamine metabolic process [GO:0042417];
elastic fiber assembly [GO:0048251];
elastin biosynthetic process [GO:0051542];
epinephrine metabolic process [GO:0042414];
extracellular matrix organization [GO:0030198];
hair follicle morphogenesis [GO:0031069];
in utero embryonic development [GO:0001701];
ion transmembrane transport [GO:0034220];
lactation [GO:0007595];
locomotory behavior [GO:0007626];
lung alveolus development [GO:0048286];
mitochondrion organization [GO:0007005];
negative regulation of catalytic activity [GO:0043086];
neuron projection morphogenesis [GO:0048812];
norepinephrine metabolic process [GO:0042415];
peptidyl-lysine modification [GO:0018205];
pigmentation [GO:0043473];
plasma membrane copper ion transport [GO:0015679];
positive regulation of catalytic activity [GO:0043085];
positive regulation of oxidoreductase activity [GO:0051353];
pyramidal neuron development [GO:0021860];
regulation of oxidative phosphorylation [GO:0002082];
removal of superoxide radicals [GO:0019430];
response to iron(III ion [GO:0010041];
response to zinc ion [GO:0010043];
serotonin metabolic process [GO:0042428];
skin development [GO:0043588];
T-helper cell differentiation [GO:0042093];
tryptophan metabolic process [GO:0006568]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
copper-dependent protein binding [GO:0032767];
copper-exporting ATPase activity [GO:0004008];
copper ion binding [GO:0005507];
copper ion transmembrane transporter activity [GO:0005375];
superoxide dismutase copper chaperone activity [GO:0016532]
Gene Ontology
(Cellular Component)
Complete annatation
basolateral plasma membrane [GO:0016323];
brush border membrane [GO:0031526];
cytosol [GO:0005829];
endoplasmic reticulum [GO:0005783];
Golgi apparatus [GO:0005794];
integral component of plasma membrane [GO:0005887];
late endosome [GO:0005770];
membrane [GO:0016020];
neuronal cell body [GO:0043025];
neuron projection [GO:0043005];
perinuclear region of cytoplasm [GO:0048471];
plasma membrane [GO:0005886];
secretory granule [GO:0030141];
trans-Golgi network [GO:0005802];
trans-Golgi network transport vesicle [GO:0030140]
Protein-protein interaction107020
Phylogenetic treeQ04656
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.454057959694761.51220004397379e-058.95203419590453e-05
AZA vs. DISU0.2938430705039480.246398249427220.829097385913675
AZA vs. IL70.512479067750860.008031482885280440.282187742493781
AZA vs. SAHA0.2190349911336160.4204967405043750.77445379597836
DISU vs. CD31.735892309644123.39134028770616e-062.8416377708106e-05
DISU vs. IL70.2094597425972690.4059617896925030.766553885946316
DISU vs. SAHA-0.07385469352071410.8019619260493250.948469804937431
DMSO vs. AZA-0.03024365228578630.8575405164103171
DMSO vs. CD31.415139783717961.54962975128514e-058.54195836191389e-05
DMSO vs. DISU-0.3252777902844360.1833691874492260.69789384340307
DMSO vs. IL70.5496440016415960.002370268752611260.0955728372419074
DMSO vs. SAHA0.2416066877064160.3809271343748760.728547314995401
HIV vs. Mock in Activation0.2120146686053750.7370601934069740.999983755607037
HIV vs. Mock in Latency0.1953061993782840.331589217904370.999834320637052
IL7 vs. CD31.974230625318813.27620586215716e-095.36180792185611e-08
SAHA vs. CD31.647800317550588.43835568953288e-065.40671854767264e-05
SAHA vs. IL7-0.296847024055620.281515858736930.528859677791685
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.406851 0.303807
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.071 1.382 1.921 1.944 1.647
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00515 Cisplatin approved unknown substrate
DB00958 Carboplatin approved unknown substrate
DB00526 Oxaliplatin approved, investigational unknown substrate

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1AW0 NMR - A=375-446.
1KVI NMR - A=1-79.
1KVJ NMR - A=1-79.
1Q8L NMR - A=164-246.
1S6O NMR - A=169-240.
1S6U NMR - A=169-240.
1Y3J NMR - A=486-558.
1Y3K NMR - A=486-558.
1YJR NMR - A=562-633.
1YJT NMR - A=562-633.
1YJU NMR - A=562-633.
1YJV NMR - A=562-633.
2AW0 NMR - A=375-446.
2G9O NMR - A=275-352.
2GA7 NMR - A=275-352.
2K1R NMR - A=5-77.
2KIJ NMR - A=806-924.
2KMV NMR - A=1051-1231.
2KMX NMR - A=1051-1231.
3CJK X-ray 1.8Å B=7-77.
5T7L X-ray 2.8Å B=7-77.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa01524 Platinum drug resistance - Homo sapiens (human)
hsa04978 Mineral absorption - Homo sapiens (human)