Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000192
UniProt IDP18859
Primary gene name(s)ATP5J
Synonym gene name(s)ATP5A, ATPM
Protein nameATP synthase-coupling factor 6, mitochondrial
Protein functionMitochondrial membrane ATP synthase, F(1F(0 ATP synthase or Complex V produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1 - containing the extramembraneous catalytic core and F(0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0 domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3beta(3 subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes.
Subcellular locationMitochondrion. Mitochondrion inner membrane.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P18859
Gene Ontology
(Biological Process)
Complete annatation
ATP biosynthetic process [GO:0006754];
mitochondrial ATP synthesis coupled proton transport [GO:0042776];
substantia nigra development [GO:0021762]
Gene Ontology
(Molecular Function)
Complete annatation
hydrogen ion transmembrane transporter activity [GO:0015078];
transmembrane transporter activity [GO:0022857];
transporter activity [GO:0005215]
Gene Ontology
(Cellular Component)
Complete annatation
mitochondrial inner membrane [GO:0005743];
mitochondrial proton-transporting ATP synthase complex [GO:0005753];
mitochondrial proton-transporting ATP synthase complex, coupling factor F(o [GO:0000276];
mitochondrion [GO:0005739]
Protein-protein interaction107006
Phylogenetic treeP18859
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.8566050404806390.01041370291427920.0269543758069752
AZA vs. DISU-0.2173351533189640.3915707611422990.901701444158113
AZA vs. IL70.2336561430871050.2261464208353320.989265982093417
AZA vs. SAHA-0.1032610375444880.6736498309935470.90439949700719
DISU vs. CD3-1.085890476086470.003609716876579180.012386267288312
DISU vs. IL70.4415893183118820.08071085220246730.376449131324301
DISU vs. SAHA0.1154861520231990.6925046402396390.909285051911964
DMSO vs. AZA-0.1380436301841950.4132038002036481
DMSO vs. CD3-1.003607643807080.002214685523475810.00681180837304269
DMSO vs. DISU0.07817196632978720.7492362457838770.969857060964769
DMSO vs. IL70.3784303699909980.0363561345634470.390919207235352
DMSO vs. SAHA0.02781114563328050.9064889577563410.977651000597239
HIV vs. Mock in Activation-0.3398401822248530.5855193373229040.999983755607037
HIV vs. Mock in Latency-0.1312622945574460.430669070667220.999834320637052
IL7 vs. CD3-0.6151390178387110.05971322077054660.12685798120852
SAHA vs. CD3-0.9840404511224470.007274987429334080.019616042326009
SAHA vs. IL7-0.3393196217954290.1658521895135580.38800356767173
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0544961 0.905272
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.068 1.067 1.277 1.267 0.965
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpu interacts with 22190034

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00190 Oxidative phosphorylation - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa05010 Alzheimer's disease - Homo sapiens (human)
hsa05012 Parkinson's disease - Homo sapiens (human)
hsa05016 Huntington's disease - Homo sapiens (human)
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