Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000189
UniProt IDP23634
Primary gene name(s)ATP2B4
Synonym gene name(s)ATP2B2, MXRA1
Protein namePlasma membrane calcium-transporting ATPase 4
Protein functionCalcium/calmodulin-regulated and magnesium-dependent enzyme that catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell, PubMed:8530416. By regulating sperm cell calcium homeostasis, may play a role in sperm motility, By similarity. {ECO:0000250|UniProtKB:Q6Q477, ECO:0000269|PubMed:8530416}.
Subcellular locationCell membrane {ECO:0000269|PubMed:2137451};
Multi-pass membrane protein {ECO:0000255}. Cell projection, cilium, flagellum membrane {ECO:0000250|UniProtKB:Q6Q477};
Multi-pass membrane protein {ECO:0000255}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P23634
Gene Ontology
(Biological Process)
Complete annatation
calcium ion export [GO:1901660];
calcium ion import across plasma membrane [GO:0098703];
calcium ion transmembrane import into cytosol [GO:0097553];
calcium ion transmembrane transport [GO:0070588];
cellular calcium ion homeostasis [GO:0006874];
cellular response to epinephrine stimulus [GO:0071872];
flagellated sperm motility [GO:0030317];
hippocampus development [GO:0021766];
ion transmembrane transport [GO:0034220];
negative regulation of adrenergic receptor signaling pathway involved in heart process [GO:1901205];
negative regulation of arginine catabolic process [GO:1900082];
negative regulation of calcineurin-NFAT signaling cascade [GO:0070885];
negative regulation of cardiac muscle hypertrophy in response to stress [GO:1903243];
negative regulation of citrulline biosynthetic process [GO:1903249];
negative regulation of nitric oxide biosynthetic process [GO:0045019];
negative regulation of nitric oxide mediated signal transduction [GO:0010751];
negative regulation of nitric-oxide synthase activity [GO:0051001];
negative regulation of peptidyl-cysteine S-nitrosylation [GO:1902083];
negative regulation of the force of heart contraction [GO:0098736];
neural retina development [GO:0003407];
positive regulation of cAMP-dependent protein kinase activity [GO:2000481];
positive regulation of peptidyl-serine phosphorylation [GO:0033138];
regulation of cardiac conduction [GO:1903779];
regulation of cell cycle G1/S phase transition [GO:1902806];
regulation of sodium ion transmembrane transport [GO:1902305];
regulation of transcription from RNA polymerase II promoter [GO:0006357];
response to hydrostatic pressure [GO:0051599];
spermatogenesis [GO:0007283]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
calcium-transporting ATPase activity [GO:0005388];
calmodulin binding [GO:0005516];
metal ion binding [GO:0046872];
nitric-oxide synthase binding [GO:0050998];
nitric-oxide synthase inhibitor activity [GO:0036487];
PDZ domain binding [GO:0030165];
protein phosphatase 2B binding [GO:0030346];
scaffold protein binding [GO:0097110]
Gene Ontology
(Cellular Component)
Complete annatation
basolateral plasma membrane [GO:0016323];
caveola [GO:0005901];
integral component of plasma membrane [GO:0005887];
intracellular membrane-bounded organelle [GO:0043231];
membrane [GO:0016020];
neuron projection [GO:0043005];
plasma membrane [GO:0005886];
protein complex [GO:0043234];
sperm flagellum [GO:0036126];
sperm principal piece [GO:0097228];
T-tubule [GO:0030315];
Z disc [GO:0030018]
Protein-protein interaction106983
Phylogenetic treeP23634
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-2.690080503393541.33226762955019e-144.35573486173111e-13
AZA vs. DISU-0.01575166892090040.9502353093730880.996649735504266
AZA vs. IL7-0.02700356557484580.8879595606119990.999311006273513
AZA vs. SAHA-0.08557385963253890.7250839924637980.923407119489742
DISU vs. CD32.661728683959046.01296790136985e-121.90819953989998e-10
DISU vs. IL7-0.02057467715228620.9348621975357920.988186499206029
DISU vs. SAHA-0.06802446175471410.8152166976681880.952413244229245
DMSO vs. AZA0.0819797292638110.6231004087276381
DMSO vs. CD32.761332926045626.66133814775094e-162.28594667872136e-14
DMSO vs. DISU0.09612935447900110.6931948955369850.957954410639349
DMSO vs. IL7-0.1018514812612710.5696979925865890.903436831021822
DMSO vs. SAHA-0.1739760927466910.4594147051065560.788579442156222
HIV vs. Mock in Activation0.559041183666680.3707213115807440.999983755607037
HIV vs. Mock in Latency0.2047111968524330.2130531571098430.999834320637052
IL7 vs. CD32.669597971813355.55111512312578e-152.17329761690584e-13
SAHA vs. CD32.580382396036245.74373881789825e-121.54018049796667e-10
SAHA vs. IL7-0.06160114836030010.7998046594151450.912937712859601
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change 1.825468076
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.42701 0.00108419
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.003 1.127 1.328 1.368 1.435
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1CFF NMR - B=1086-1104.
2KNE NMR - B=1086-1149.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04020 Calcium signaling pathway - Homo sapiens (human)
hsa04022 cGMP-PKG signaling pathway - Homo sapiens (human)
hsa04024 cAMP signaling pathway - Homo sapiens (human)
hsa04261 Adrenergic signaling in cardiomyocytes - Homo sapiens (human)
hsa04970 Salivary secretion - Homo sapiens (human)
hsa04972 Pancreatic secretion - Homo sapiens (human)