Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000187
UniProt IDP16615
Primary gene name(s)ATP2A2
Synonym gene name(s)ATP2B
Protein nameSarcoplasmic/endoplasmic reticulum calcium ATPase 2
Protein functionThis magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle, PubMed:16402920. Acts as a regulator of TNFSF11-mediated Ca(2+ signaling pathways via its interaction with TMEM64 which is critical for the TNFSF11-induced CREB1 activation and mitochondrial ROS generation necessary for proper osteoclast generation. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca, 2+ spiking for activation of NFATC1 and production of mitochondrial ROS, thereby triggering Ca, 2+ signaling cascades that promote osteoclast differentiation and activation, By similarity. {ECO:0000250|UniProtKB:O55143, ECO:0000269|PubMed:16402920}.
Subcellular locationEndoplasmic reticulum membrane {ECO:0000250|UniProtKB:O55143};
Multi-pass membrane protein {ECO:0000255}. Sarcoplasmic reticulum membrane {ECO:0000250|UniProtKB:O55143};
Multi-pass membrane protein {ECO:0000255}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P16615
Gene Ontology
(Biological Process)
Complete annatation
calcium ion import into sarcoplasmic reticulum [GO:1990036];
calcium ion transmembrane transport [GO:0070588];
calcium ion transport from cytosol to endoplasmic reticulum [GO:1903515];
cardiac muscle hypertrophy in response to stress [GO:0014898];
cell adhesion [GO:0007155];
cellular calcium ion homeostasis [GO:0006874];
cellular response to oxidative stress [GO:0034599];
endoplasmic reticulum calcium ion homeostasis [GO:0032469];
epidermis development [GO:0008544];
ER-nucleus signaling pathway [GO:0006984];
ion transmembrane transport [GO:0034220];
negative regulation of heart contraction [GO:0045822];
organelle organization [GO:0006996];
positive regulation of endoplasmic reticulum calcium ion concentration [GO:0032470];
positive regulation of heart rate [GO:0010460];
regulation of calcium ion-dependent exocytosis of neurotransmitter [GO:1903233];
regulation of cardiac conduction [GO:1903779];
regulation of cardiac muscle cell action potential involved in regulation of contraction [GO:0098909];
regulation of cardiac muscle cell membrane potential [GO:0086036];
regulation of cardiac muscle contraction by calcium ion signaling [GO:0010882];
regulation of the force of heart contraction [GO:0002026];
relaxation of cardiac muscle [GO:0055119];
response to endoplasmic reticulum stress [GO:0034976];
response to peptide hormone [GO:0043434];
sarcoplasmic reticulum calcium ion transport [GO:0070296];
transition between fast and slow fiber [GO:0014883];
T-tubule organization [GO:0033292]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
calcium ion binding [GO:0005509];
calcium-transporting ATPase activity [GO:0005388];
calcium-transporting ATPase activity involved in regulation of cardiac muscle cell membrane potential [GO:0086039];
enzyme binding [GO:0019899];
protein C-terminus binding [GO:0008022];
S100 protein binding [GO:0044548]
Gene Ontology
(Cellular Component)
Complete annatation
calcium ion-transporting ATPase complex [GO:0090534];
endoplasmic reticulum [GO:0005783];
endoplasmic reticulum membrane [GO:0005789];
extrinsic component of cytoplasmic side of plasma membrane [GO:0031234];
integral component of plasma membrane [GO:0005887];
longitudinal sarcoplasmic reticulum [GO:0014801];
membrane [GO:0016020];
perinuclear region of cytoplasm [GO:0048471];
platelet dense tubular network membrane [GO:0031095];
protein complex [GO:0043234];
ribbon synapse [GO:0097470];
sarcoplasmic reticulum [GO:0016529];
sarcoplasmic reticulum membrane [GO:0033017];
vesicle membrane [GO:0012506]
Protein-protein interaction106978
Phylogenetic treeP16615
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.136240416328450.0005958692290858330.0023170642468477
AZA vs. DISU0.1949047709701560.4418876299064530.91814655876178
AZA vs. IL70.2881163878209550.1334158456085260.898662602392564
AZA vs. SAHA-0.3871716494593080.1126526371598860.428563313273369
DISU vs. CD3-0.9542128261955370.009236556422017040.0271037281497927
DISU vs. IL70.08424707510075350.738489653063030.935156881173568
DISU vs. SAHA-0.5802748892945750.04710486640376940.270238843384549
DMSO vs. AZA0.04833277632942380.7724540453276841
DMSO vs. CD3-1.100336153544220.0006572599481440290.00236613742186364
DMSO vs. DISU-0.1487160371293410.5425755700432830.921839163510307
DMSO vs. IL70.247285846238630.1683202358060980.677338767617689
DMSO vs. SAHA-0.441516555182010.06123898370994120.283988328253667
HIV vs. Mock in Activation0.3071645903479630.6223994557941870.999983755607037
HIV vs. Mock in Latency-0.1068107851343510.5162708684897810.999834320637052
IL7 vs. CD3-0.841022997349310.009185373461934380.0277735474807897
SAHA vs. CD3-1.547628363692911.80765442945319e-050.000105504849929743
SAHA vs. IL7-0.6784242711779540.005486467985076020.0412483882329356
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.583649 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.997 1.057 1.157 1.159 0.985
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Rev interacts with 22174317
Envelope surface glycoprotein gp160; precursor interacts with 22190034

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04020 Calcium signaling pathway - Homo sapiens (human)
hsa04022 cGMP-PKG signaling pathway - Homo sapiens (human)
hsa04024 cAMP signaling pathway - Homo sapiens (human)
hsa04260 Cardiac muscle contraction - Homo sapiens (human)
hsa04261 Adrenergic signaling in cardiomyocytes - Homo sapiens (human)
hsa04919 Thyroid hormone signaling pathway - Homo sapiens (human)
hsa04972 Pancreatic secretion - Homo sapiens (human)
hsa05010 Alzheimer's disease - Homo sapiens (human)
hsa05410 Hypertrophic cardiomyopathy (HCM) - Homo sapiens (human)
hsa05412 Arrhythmogenic right ventricular cardiomyopathy (ARVC) - Homo sapiens (human)
hsa05414 Dilated cardiomyopathy - Homo sapiens (human)