Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000186
UniProt IDP54709
Primary gene name(s)ATP1B3
Synonym gene name(s)unknown
Protein nameSodium/potassium-transporting ATPase subunit beta-3
Protein functionThis is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+ and K(+ ions across the plasma membrane. The exact function of the beta-3 subunit is not known.
Subcellular locationCell membrane {ECO:0000269|PubMed:17081065};
Single-pass type II membrane protein {ECO:0000269|PubMed:17081065}. Melanosome {ECO:0000269|PubMed:17081065}. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P54709
Gene Ontology
(Biological Process)
Complete annatation
cellular potassium ion homeostasis [GO:0030007];
cellular sodium ion homeostasis [GO:0006883];
ion transmembrane transport [GO:0034220];
leukocyte migration [GO:0050900];
membrane repolarization [GO:0086009];
positive regulation of ATPase activity [GO:0032781];
positive regulation of potassium ion import [GO:1903288];
positive regulation of potassium ion transmembrane transporter activity [GO:1901018];
positive regulation of sodium ion export from cell [GO:1903278];
potassium ion import [GO:0010107];
protein localization to plasma membrane [GO:0072659];
protein stabilization [GO:0050821];
regulation of cardiac conduction [GO:1903779];
sodium ion export from cell [GO:0036376];
transport [GO:0006810]
Gene Ontology
(Molecular Function)
Complete annatation
ATPase activator activity [GO:0001671];
ATPase binding [GO:0051117];
sodium:potassium-exchanging ATPase activity [GO:0005391]
Gene Ontology
(Cellular Component)
Complete annatation
caveola [GO:0005901];
extracellular exosome [GO:0070062];
melanosome [GO:0042470];
plasma membrane [GO:0005886];
sodium:potassium-exchanging ATPase complex [GO:0005890]
Protein-protein interaction106973
Phylogenetic treeP54709
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD32.47340513459342.30512184895559e-082.5194196162044e-07
AZA vs. DISU0.3244335565834330.2019273778449270.791291305631523
AZA vs. IL70.2838073604705710.1405358614799570.905250409850298
AZA vs. SAHA-0.01378085346807940.9550088174179530.99052408598402
DISU vs. CD3-2.162115286979015.86548356662497e-075.99432537513931e-06
DISU vs. IL7-0.04932387612820840.8450800367267070.96804956582425
DISU vs. SAHA-0.3366879175394030.2526767827777170.631210308841866
DMSO vs. AZA-0.07838257370931490.6406256691486661
DMSO vs. CD3-2.563894916075577.05321034910611e-097.76729633839792e-08
DMSO vs. DISU-0.4047925490764840.09899096017243390.568518967148849
DMSO vs. IL70.3695379794731840.04032821225871490.408764355963649
DMSO vs. SAHA0.05822625691130760.8051977150451870.945766874245656
HIV vs. Mock in Activation-0.2610024958414650.7742202015543450.999983755607037
HIV vs. Mock in Latency-0.05339976493193840.7471208234828840.999834320637052
IL7 vs. CD3-2.181387381567525.07773191604777e-075.30032224173047e-06
SAHA vs. CD3-2.511994368934155.90436411984641e-086.57154854402403e-07
SAHA vs. IL7-0.3010268229460990.2178433417940440.45626935309204
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.419156 0.00132191
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.027 1.05 1.003 1.081 1.104
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04022 cGMP-PKG signaling pathway - Homo sapiens (human)
hsa04024 cAMP signaling pathway - Homo sapiens (human)
hsa04260 Cardiac muscle contraction - Homo sapiens (human)
hsa04261 Adrenergic signaling in cardiomyocytes - Homo sapiens (human)
hsa04911 Insulin secretion - Homo sapiens (human)
hsa04918 Thyroid hormone synthesis - Homo sapiens (human)
hsa04919 Thyroid hormone signaling pathway - Homo sapiens (human)
hsa04960 Aldosterone-regulated sodium reabsorption - Homo sapiens (human)
hsa04961 Endocrine and other factor-regulated calcium reabsorption - Homo sapiens (human)
hsa04964 Proximal tubule bicarbonate reclamation - Homo sapiens (human)
hsa04970 Salivary secretion - Homo sapiens (human)
hsa04971 Gastric acid secretion - Homo sapiens (human)
hsa04972 Pancreatic secretion - Homo sapiens (human)
hsa04973 Carbohydrate digestion and absorption - Homo sapiens (human)
hsa04974 Protein digestion and absorption - Homo sapiens (human)
hsa04976 Bile secretion - Homo sapiens (human)
hsa04978 Mineral absorption - Homo sapiens (human)