Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000185
UniProt IDP05023
Primary gene name(s)ATP1A1
Synonym gene name(s)unknown
Protein nameSodium/potassium-transporting ATPase subunit alpha-1
Protein functionThis is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Subcellular locationCell membrane {ECO:0000269|PubMed:7711835};
Multi-pass membrane protein {ECO:0000255}. Melanosome {ECO:0000269|PubMed:17081065}. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. {ECO:0000269|PubMed:17081065}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P05023
Gene Ontology
(Biological Process)
Complete annatation
ATP hydrolysis coupled proton transport [GO:0015991];
cardiac muscle cell action potential involved in contraction [GO:0086002];
cell communication by electrical coupling involved in cardiac conduction [GO:0086064];
cellular potassium ion homeostasis [GO:0030007];
cellular response to mechanical stimulus [GO:0071260];
cellular response to steroid hormone stimulus [GO:0071383];
cellular sodium ion homeostasis [GO:0006883];
ion transmembrane transport [GO:0034220];
membrane hyperpolarization [GO:0060081];
membrane repolarization [GO:0086009];
membrane repolarization during cardiac muscle cell action potential [GO:0086013];
negative regulation of glucocorticoid biosynthetic process [GO:0031947];
negative regulation of heart contraction [GO:0045822];
positive regulation of heart contraction [GO:0045823];
positive regulation of striated muscle contraction [GO:0045989];
potassium ion import [GO:0010107];
potassium ion import across plasma membrane [GO:1990573];
regulation of blood pressure [GO:0008217];
regulation of cardiac conduction [GO:1903779];
regulation of cardiac muscle cell contraction [GO:0086004];
regulation of sodium ion transport [GO:0002028];
regulation of the force of heart contraction [GO:0002026];
relaxation of cardiac muscle [GO:0055119];
response to drug [GO:0042493];
response to glycoside [GO:1903416];
sodium ion export from cell [GO:0036376]
Gene Ontology
(Molecular Function)
Complete annatation
ADP binding [GO:0043531];
ATP binding [GO:0005524];
chaperone binding [GO:0051087];
phosphatase activity [GO:0016791];
potassium ion binding [GO:0030955];
sodium:potassium-exchanging ATPase activity [GO:0005391];
sodium ion binding [GO:0031402];
steroid hormone binding [GO:1990239]
Gene Ontology
(Cellular Component)
Complete annatation
apical plasma membrane [GO:0016324];
basolateral plasma membrane [GO:0016323];
caveola [GO:0005901];
endoplasmic reticulum [GO:0005783];
endosome [GO:0005768];
extracellular exosome [GO:0070062];
extracellular vesicle [GO:1903561];
Golgi apparatus [GO:0005794];
integral component of membrane [GO:0016021];
intercalated disc [GO:0014704];
intracellular membrane-bounded organelle [GO:0043231];
melanosome [GO:0042470];
membrane [GO:0016020];
myelin sheath [GO:0043209];
neuronal postsynaptic density [GO:0097481];
plasma membrane [GO:0005886];
protein complex [GO:0043234];
sarcolemma [GO:0042383];
sodium:potassium-exchanging ATPase complex [GO:0005890];
T-tubule [GO:0030315]
Protein-protein interaction106966
Phylogenetic treeP05023
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.2675803439345950.4186703298631840.540377323303953
AZA vs. DISU-0.2637034923493470.2963474336406870.859528450507179
AZA vs. IL70.153311888146140.4236931852020190.999311006273513
AZA vs. SAHA-0.01942942516347460.9363520657803780.986412121012888
DISU vs. CD3-0.5438738306839930.1391306785442410.241690667170826
DISU vs. IL70.4083167825554820.1047707555073150.426359055630369
DISU vs. SAHA0.2454230526959870.3989617347822060.759591926150641
DMSO vs. AZA0.004801465175197770.9770389371174651
DMSO vs. CD3-0.2768872705798070.3924622006567870.506940157154633
DMSO vs. DISU0.2658162610666540.2751108746654980.793994270556585
DMSO vs. IL70.1562514356994170.3832459434836240.835709304187443
DMSO vs. SAHA-0.03058999585779840.8965106070657630.975026503245809
HIV vs. Mock in Activation-0.09334939616224930.889546281966390.999983755607037
HIV vs. Mock in Latency-0.1712053544514970.2980689250535420.999834320637052
IL7 vs. CD3-0.1058561664146290.7447221222518290.829319236048841
SAHA vs. CD3-0.3122882223258830.3811542118516060.497703316229417
SAHA vs. IL7-0.1766237103682660.4673121815335680.702236310538316
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.150482 0.904387
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.952 1.009 1.067 1.098 1.117
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00390 Digoxin approved yes unknown
DB00511 Acetyldigitoxin approved yes inhibitor
DB01078 Deslanoside approved yes inhibitor
DB01092 Ouabain approved yes inhibitor
DB00774 Hydroflumethiazide approved unknown other/unknown
DB01021 Trichlormethiazide approved, vet_approved yes inhibitor
DB01396 Digitoxin approved yes inhibitor
DB00903 Etacrynic acid approved yes inhibitor
DB01119 Diazoxide approved unknown other
DB01158 Bretylium approved yes inhibitor
DB01244 Bepridil approved, withdrawn unknown inhibitor
DB01345 Potassium approved unknown unknown
DB01370 Aluminium approved unknown binder
DB01378 Magnesium nutraceutical unknown unknown
DB01430 Almitrine approved yes binder
DB01188 Ciclopirox approved, investigational yes binder

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Pr55(Gag) incorporates 25631074
Envelope surface glycoprotein gp120 complexes with 23125841
Gag-Pol complexes with 23125841
Pr55(Gag) complexes with 23125841
Pr55(Gag) fractionates with 25416948
Nef complexes with 23125841

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04022 cGMP-PKG signaling pathway - Homo sapiens (human)
hsa04024 cAMP signaling pathway - Homo sapiens (human)
hsa04260 Cardiac muscle contraction - Homo sapiens (human)
hsa04261 Adrenergic signaling in cardiomyocytes - Homo sapiens (human)
hsa04911 Insulin secretion - Homo sapiens (human)
hsa04918 Thyroid hormone synthesis - Homo sapiens (human)
hsa04919 Thyroid hormone signaling pathway - Homo sapiens (human)
hsa04960 Aldosterone-regulated sodium reabsorption - Homo sapiens (human)
hsa04961 Endocrine and other factor-regulated calcium reabsorption - Homo sapiens (human)
hsa04964 Proximal tubule bicarbonate reclamation - Homo sapiens (human)
hsa04970 Salivary secretion - Homo sapiens (human)
hsa04971 Gastric acid secretion - Homo sapiens (human)
hsa04972 Pancreatic secretion - Homo sapiens (human)
hsa04973 Carbohydrate digestion and absorption - Homo sapiens (human)
hsa04974 Protein digestion and absorption - Homo sapiens (human)
hsa04976 Bile secretion - Homo sapiens (human)
hsa04978 Mineral absorption - Homo sapiens (human)