Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000183
UniProt IDP31939
Primary gene name(s)ATIC
Synonym gene name(s)PURH
Protein nameBifunctional purine biosynthesis protein PURH [Cleaved into: Bifunctional purine biosynthesis protein PURH, N-terminally processed] [Includes: Phosphoribosylaminoimidazolecarboxamide formyltransferase
Protein functionBifunctional enzyme that catalyzes 2 steps in purine biosynthesis. {ECO:0000269|PubMed:14966129}.; FUNCTION: Promotes insulin receptor/INSR autophosphorylation and is involved in INSR internalization, PubMed:25687571. {ECO:0000269|PubMed:25687571}.
Subcellular locationunknown
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P31939
Gene Ontology
(Biological Process)
Complete annatation
'de novo' IMP biosynthetic process [GO:0006189];
animal organ regeneration [GO:0031100];
brainstem development [GO:0003360];
cerebellum development [GO:0021549];
cerebral cortex development [GO:0021987];
dihydrofolate metabolic process [GO:0046452];
nucleobase-containing compound metabolic process [GO:0006139];
nucleoside metabolic process [GO:0009116];
purine ribonucleoside monophosphate biosynthetic process [GO:0009168];
response to inorganic substance [GO:0010035];
tetrahydrofolate biosynthetic process [GO:0046654]
Gene Ontology
(Molecular Function)
Complete annatation
IMP cyclohydrolase activity [GO:0003937];
phosphoribosylaminoimidazolecarboxamide formyltransferase activity [GO:0004643];
protein homodimerization activity [GO:0042803]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
membrane [GO:0016020];
mitochondrion [GO:0005739]
Protein-protein interaction106961
Phylogenetic treeP31939
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.389108835193553.10355864592271e-050.000171658113436136
AZA vs. DISU-0.2290540975081810.3658692251307380.896211275303082
AZA vs. IL70.4698514678044490.02299623897611990.482089443792845
AZA vs. SAHA-0.5194225823343330.03511225421055340.218350709558532
DISU vs. CD3-1.629876491761471.17683508091826e-058.603109607062e-05
DISU vs. IL70.6899143360324980.006445370389817740.087772320349144
DISU vs. SAHA-0.2891566758769420.3264773525618580.708880590759221
DMSO vs. AZA-0.006777439081043580.9702008064542441
DMSO vs. CD3-1.407493944382851.53751060764096e-058.49068816133102e-05
DMSO vs. DISU0.2203765141858120.3673577132462480.850877793209398
DMSO vs. IL70.4839932774286980.008682058405230950.193425089402849
DMSO vs. SAHA-0.5190893547004330.02900887305178360.180392578896949
HIV vs. Mock in Activation-0.2149179198845350.7295704833507920.999983755607037
HIV vs. Mock in Latency-0.05814392860253370.7259518563317080.999834320637052
IL7 vs. CD3-0.9112095440707350.004852513874104350.0163372663891836
SAHA vs. CD3-1.932865611757731.268817811928e-071.29722418085176e-06
SAHA vs. IL7-0.9923639249853745.90413445631377e-050.00127872015509301
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
-0.426 0.03047

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0507433 0.79153
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.003 0.951 0.928 0.893 0.876
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB01700 Aicar experimental unknown unknown
DB01972 Guanosine-5&,39;-Monophosphate experimental unknown unknown
DB02309 5--Monophosphate-9-Beta-D-Ribofuranosyl Xanthine experimental unknown unknown
DB03442 2-[5-Hydroxy-3-Methyl-1-(2-Methyl-4-Sulfo-Phenyl)-1h-Pyrazol-4-Ylazo]-4-Sulfo-Benzoic Acid experimental unknown unknown
DB04057 Beta-Dadf, Msa, Multisubstrate Adduct Inhibitor experimental unknown unknown
DB00116 Tetrahydrofolic acid approved, nutraceutical unknown cofactor
DB00642 Pemetrexed approved, investigational yes inhibitor
DB00563 Methotrexate approved unknown inhibitor

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1P4R X-ray 2.5Å A/B=1-592.
1PKX X-ray 1.9Å A/B/C/D=1-592.
1PL0 X-ray 2.6Å A/B/C/D=1-592.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00230 Purine metabolism - Homo sapiens (human)
hsa00670 One carbon pool by folate - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa01523 Antifolate resistance - Homo sapiens (human)