Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000171
UniProt IDP04424
Primary gene name(s)ASL
Synonym gene name(s)unknown
Protein nameArgininosuccinate lyase
Protein functionunknown
Subcellular locationunknown
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P04424
Gene Ontology
(Biological Process)
Complete annatation
arginine biosynthetic process [GO:0006526];
arginine biosynthetic process via ornithine [GO:0042450];
arginine catabolic process [GO:0006527];
internal protein amino acid acetylation [GO:0006475];
protein tetramerization [GO:0051262];
urea cycle [GO:0000050]
Gene Ontology
(Molecular Function)
Complete annatation
argininosuccinate lyase activity [GO:0004056]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
extracellular exosome [GO:0070062]
Protein-protein interaction106927
Phylogenetic treeP04424
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: HCV; HCV(r
      Viral life cycle: translation
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.1787301462063140.7756336765322250.845167059911343
AZA vs. DISU-0.8063707923713180.005876850541704280.199890674344511
AZA vs. IL7-0.0124137872636230.9592880407863210.999311006273513
AZA vs. SAHA-0.2163710308184170.4139669943640570.768868712794948
DISU vs. CD3-0.640024458929320.3495991008409450.483317476301763
DISU vs. IL70.7832237381267890.009376481889025960.112899732010436
DISU vs. SAHA0.5927488190879850.0619624969248420.315306677915427
DMSO vs. AZA0.04301328706673450.8194728515829611
DMSO vs. CD30.2103097376858860.7306641221910040.804403317802359
DMSO vs. DISU0.8472515137473390.0009017924026614170.0631996663742576
DMSO vs. IL7-0.04785512947719030.8109438766946950.962475569829797
DMSO vs. SAHA-0.2635057128904860.2719041908134390.621692627313963
HIV vs. Mock in Activation0.3833257206928630.7699665575940880.999983755607037
HIV vs. Mock in Latency0.2331269563099520.1706518256981380.999834320637052
IL7 vs. CD30.1702807999089270.7835843614003330.856863144687763
SAHA vs. CD3-0.0605354806854210.9236943117041990.94695704220874
SAHA vs. IL7-0.2053424768423270.4528797880430470.690841049724825
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
1.8 0.001899263 1.3 0.096597953 1.6 0.010280366
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 1.52666 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.99 0.89 0.888 1.067 0.842
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00125 L-Arginine approved, nutraceutical unknown unknown
DB02267 Argininosuccinate experimental unknown unknown
DB03814 2-(N-Morpholino)-Ethanesulfonic Acid experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1AOS X-ray 4.2Å A/B=1-464.
1K62 X-ray 2.6Å A/B=1-464.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00220 Arginine biosynthesis - Homo sapiens (human)
hsa00250 Alanine# aspartate and glutamate metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa01230 Biosynthesis of amino acids - Homo sapiens (human)