Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000170
UniProt IDQ9NR48
Primary gene name(s)ASH1L
Synonym gene name(s)KIAA1420, KMT2H
Protein nameHistone-lysine N-methyltransferase ASH1L
Protein functionHistone methyltransferase specifically methylating 'Lys-36' of histone H3, H3K36me. {ECO:0000269|PubMed:21239497}.
Subcellular locationNucleus {ECO:0000269|PubMed:10860993}. Cell junction, tight junction {ECO:0000269|PubMed:10860993}. Chromosome {ECO:0000305|PubMed:10860993}. Note=The relevance of tight junction localization is however unclear.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9NR48
Gene Ontology
(Biological Process)
Complete annatation
cell-cell signaling [GO:0007267];
DNA packaging [GO:0006323];
histone H3-K36 dimethylation [GO:0097676];
interleukin-6 production [GO:0032635];
negative regulation of acute inflammatory response [GO:0002674];
negative regulation of I-kappaB kinase/NF-kappaB signaling [GO:0043124];
negative regulation of MAPK cascade [GO:0043409];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
transcription from RNA polymerase II promoter [GO:0006366]
Gene Ontology
(Molecular Function)
Complete annatation
chromatin binding [GO:0003682];
DNA binding [GO:0003677];
histone methyltransferase activity, H3-K36 specific [GO:0046975];
histone methyltransferase activity, H3-K4 specific [GO:0042800];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
bicellular tight junction [GO:0005923];
chromosome [GO:0005694];
cytoplasm [GO:0005737];
extracellular exosome [GO:0070062];
Golgi apparatus [GO:0005794];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction120969
Phylogenetic treeQ9NR48
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.5167665421044950.1186272335248320.201822966890875
AZA vs. DISU0.2960383539876560.2421857274597280.826119378754298
AZA vs. IL70.01316145081780780.9453235965918950.999311006273513
AZA vs. SAHA-0.1432540147778270.5565387679977550.849328908215548
DISU vs. CD30.7994300799318830.03134542238962490.07414104771712
DISU vs. IL7-0.2917196298742490.2472994417056240.63340683517948
DISU vs. SAHA-0.4379483056435850.1334026164146090.469295432005266
DMSO vs. AZA0.04256189087411170.7990375431127891
DMSO vs. CD30.5477141990291950.0905021773151520.157511009848979
DMSO vs. DISU-0.2554093283681590.2953701655720970.806677299793064
DMSO vs. IL7-0.02209085983834080.9020370624121740.979228917441583
DMSO vs. SAHA-0.1927903838612910.4129250606130460.753878332534322
HIV vs. Mock in Activation0.2984535934700520.6909446866167090.999983755607037
HIV vs. Mock in Latency0.02435928057600220.8823319284594720.999834320637052
IL7 vs. CD30.5375430876715270.09807271482199160.18690732463167
SAHA vs. CD30.348366375014120.3292892899635080.444618725898224
SAHA vs. IL7-0.1604137285031240.5095296942643640.734638987215784
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.33963 0.030899
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3MQM X-ray 2.5Å A/B=2438-2561.
3OPE X-ray 2.9Å A/B=2074-2293.
4YNM X-ray 2.1Å A/B=2074-2293.
4YNP X-ray 2.9Å A/B=2074-2293.
4YPA X-ray 2.3Å A/B/C/D=2074-2293.
4YPE X-ray 2.2Å A/B=2074-2293.
4YPU X-ray 2.6Å A/B=2074-2293.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat downregulates 22632162

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00310 Lysine degradation - Homo sapiens (human)
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