Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000160
UniProt IDQ96B67
Primary gene name(s)ARRDC3
Synonym gene name(s)KIAA1376
Protein nameArrestin domain-containing protein 3
Protein functionAdapter protein that plays a role in regulating cell-surface expression of adrenergic receptors and probably also other G protein-coupled receptors, PubMed:20559325, PubMed:21982743, PubMed:23208550. Plays a role in NEDD4-mediated ubiquitination and endocytosis af activated ADRB2 and subsequent ADRB2 degradation, PubMed:20559325, PubMed:23208550. May recruit NEDD4 to ADRB2, PubMed:20559325. Alternatively, may function as adapter protein that does not play a major role in recruiting NEDD4 to ADRB2, but rather plays a role in a targeting ADRB2 to endosomes, PubMed:23208550. {ECO:0000269|PubMed:20559325, ECO:0000269|PubMed:23208550}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:16269462, ECO:0000269|PubMed:23208550}. Cell membrane {ECO:0000269|PubMed:16269462, ECO:0000269|PubMed:20559325, ECO:0000269|PubMed:23208550};
Peripheral membrane protein {ECO:0000269|PubMed:16269462, ECO:0000269|PubMed:20559325};
Cytoplasmic side {ECO:0000269|PubMed:16269462, ECO:0000269|PubMed:20559325}. Lysosome {ECO:0000269|PubMed:16269462, ECO:0000269|PubMed:23208550}. Endosome {ECO:0000269|PubMed:16269462, ECO:0000269|PubMed:23208550}. Early endosome {ECO:0000269|PubMed:20559325, ECO:0000269|PubMed:23208550}. Note=Associated with plasma membrane, as well as with endosomes and lysosomes during endocytosis, PubMed:16269462, PubMed:23208550, PubMed:20559325. {ECO:0000269|PubMed:16269462, ECO:0000269|PubMed:20559325, ECO:0000269|PubMed:23208550}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q96B67
Gene Ontology
(Biological Process)
Complete annatation
fat pad development [GO:0060613];
negative regulation of heat generation [GO:0031651];
negative regulation of locomotion involved in locomotory behavior [GO:0090327];
negative regulation of multicellular organismal metabolic process [GO:0044252];
positive regulation of adrenergic receptor signaling pathway [GO:0071879];
positive regulation of ubiquitin-protein transferase activity [GO:0051443];
signal transduction [GO:0007165];
skin development [GO:0043588];
temperature homeostasis [GO:0001659]
Gene Ontology
(Molecular Function)
Complete annatation
beta-3 adrenergic receptor binding [GO:0031699]
Gene Ontology
(Cellular Component)
Complete annatation
early endosome [GO:0005769];
endosome [GO:0005768];
lysosome [GO:0005764];
plasma membrane [GO:0005886]
Protein-protein interaction121616
Phylogenetic treeQ96B67
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.189934274728980.02724224340105560.0600922560524326
AZA vs. DISU0.3360693891631870.5711543875796930.954304256505839
AZA vs. IL70.1902012772362880.7022022059736440.999311006273513
AZA vs. SAHA0.1874499325504050.6977862695271870.914914296883916
DISU vs. CD31.510693941946280.01269708047550140.035463366886735
DISU vs. IL7-0.152761638267750.7980151282696320.955213923328671
DISU vs. SAHA-0.1492486676353630.7944510167492120.945697774910246
DMSO vs. AZA-0.1048525470628480.8151260835070691
DMSO vs. CD31.064895411478910.02641038479468990.0564678465699693
DMSO vs. DISU-0.4453720255189480.4153994307875350.8747838183629
DMSO vs. IL70.3035152285066990.4972157968376350.885687081722074
DMSO vs. SAHA0.2844167035684180.5069851770127260.818074429013708
HIV vs. Mock in Activation-0.9828204465864610.1213670951460550.999983755607037
HIV vs. Mock in Latency-0.1251422370653890.4500165383501020.999834320637052
IL7 vs. CD31.393669500859160.0113242652781730.0330219869487138
SAHA vs. CD31.347465780064820.009764864943032410.0251334781710501
SAHA vs. IL7-0.0109224601733330.9819675372090690.993701574643495
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock -0.806 1.62E-05 2.63E-04
Infected vs. Bystander -1.17 5.10E-08 1.45E-06
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change 1.791589781
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.134266 0.391289
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4N7H X-ray 1.7Å B=342-354.
4R7V X-ray 1.7Å A=1-165.
4R7X X-ray 2.6Å A/B=1-180.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found