Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000151
UniProt IDP56559
Primary gene name(s)ARL4C
Synonym gene name(s)ARL7
Protein nameADP-ribosylation factor-like protein 4C
Protein functionSmall GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors, GEF and GTPase-activating proteins, GAP. GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. May be involved in transport between a perinuclear compartment and the plasma membrane, apparently linked to the ABCA1-mediated cholesterol secretion pathway. Recruits CYTH1, CYTH2, CYTH3 and CYTH4 to the plasma membrane in the GDP-bound form. Regulates the microtubule-dependent intracellular vesicular transport from early endosome to recycling endosome process. {ECO:0000269|PubMed:15147902, ECO:0000269|PubMed:17398095, ECO:0000269|PubMed:19409876}.
Subcellular locationCell projection, filopodium. Cell membrane. Cytoplasm.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P56559
Gene Ontology
(Biological Process)
Complete annatation
endocytic recycling [GO:0032456];
small GTPase mediated signal transduction [GO:0007264]
Gene Ontology
(Molecular Function)
Complete annatation
alpha-tubulin binding [GO:0043014];
GTPase activity [GO:0003924];
GTP binding [GO:0005525]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
filopodium [GO:0030175];
nucleus [GO:0005634];
plasma membrane [GO:0005886]
Protein-protein interaction115427
Phylogenetic treeP56559
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-2.9807621883976600
AZA vs. DISU-0.298056212902930.2377885913936690.821854758571361
AZA vs. IL70.1421742233925160.4578964509600890.999311006273513
AZA vs. SAHA-0.3914606135950160.1099677824760050.422332319246699
DISU vs. CD32.670117248557335.18407539118471e-121.66619941250998e-10
DISU vs. IL70.4312011561809620.08673471192152020.389337493542563
DISU vs. SAHA-0.09163988593528760.7541388550536510.930658150857737
DMSO vs. AZA-0.02555083718402080.87817116602581
DMSO vs. CD32.9439023720595800
DMSO vs. DISU0.2708596112922290.2660279557045310.784825275834553
DMSO vs. IL70.1749609525913420.3286467920271720.80304552824776
DMSO vs. SAHA-0.3719289099658110.1156889891229670.404441836017684
HIV vs. Mock in Activation0.2284551082541740.7135748434032820.999983755607037
HIV vs. Mock in Latency0.168178669594540.3057707514426760.999834320637052
IL7 vs. CD33.129991892235800
SAHA vs. CD32.565709096293536.58850751733553e-121.74804239940575e-10
SAHA vs. IL7-0.5364215731981720.0281816008796210.124943956370414
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.226074 0.107341
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.808 1.13 0.708 0.484 1.149
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category