Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000147
UniProt IDQ9Y4X5
Primary gene name(s)ARIH1
Synonym gene name(s)ARI, MOP6, UBCH7BP
Protein nameE3 ubiquitin-protein ligase ARIH1
Protein functionE3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3, PubMed:15236971, PubMed:21532592, PubMed:24076655, PubMed:27565346, PubMed:23707686. Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-RING ubiquitin ligase, CRL complexes and initiating ubiquitination of CRL substrates: associates with CRL complexes and specifically mediates addition of the first ubiquitin on CRLs targets, PubMed:27565346. The initial ubiquitin is then elongated by CDC34/UBE2R1 and UBE2R2, PubMed:27565346. E3 ubiquitin-protein ligase activity is activated upon binding to neddylated cullin-RING ubiquitin ligase complexes, PubMed:24076655, PubMed:27565346. Plays a role in protein translation in response to DNA damage by mediating ubiquitination of EIF4E2, the consequences of EIF4E2 ubiquitination are however unclear, PubMed:25624349. According to a report, EIF4E2 ubiquitination leads to promote EIF4E2 cap-binding and protein translation arrest, PubMed:25624349. According to another report EIF4E2 ubiquitination leads to its subsequent degradation, PubMed:14623119. Acts as the ligase involved in ISGylation of EIF4E2, PubMed:17289916. {ECO:0000269|PubMed:14623119, ECO:0000269|PubMed:15236971, ECO:0000269|PubMed:17289916, ECO:0000269|PubMed:21532592, ECO:0000269|PubMed:23707686, ECO:0000269|PubMed:24076655, ECO:0000269|PubMed:25624349, ECO:0000269|PubMed:27565346}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:11278816, ECO:0000269|PubMed:21590270, ECO:0000269|PubMed:23059369}. Nucleus {ECO:0000269|PubMed:23059369}. Nucleus, Cajal body {ECO:0000269|PubMed:23059369}. Note=Mainly cytoplasmic, PubMed:11278816. Present in Lewy body, PubMed:21590270. {ECO:0000269|PubMed:11278816, ECO:0000269|PubMed:21590270}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9Y4X5
Gene Ontology
(Biological Process)
Complete annatation
positive regulation of proteasomal ubiquitin-dependent protein catabolic process [GO:0032436];
protein polyubiquitination [GO:0000209];
protein ubiquitination [GO:0016567];
protein ubiquitination involved in ubiquitin-dependent protein catabolic process [GO:0042787];
ubiquitin-dependent protein catabolic process [GO:0006511]
Gene Ontology
(Molecular Function)
Complete annatation
ligase activity [GO:0016874];
ubiquitin conjugating enzyme binding [GO:0031624];
ubiquitin-like protein transferase activity [GO:0019787];
ubiquitin protein ligase activity [GO:0061630];
ubiquitin protein ligase binding [GO:0031625];
ubiquitin-protein transferase activity [GO:0004842];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
ubiquitin ligase complex [GO:0000151]
Protein-protein interaction117348
Phylogenetic treeQ9Y4X5
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.1670377064673580.6099992276347950.713258609276229
AZA vs. DISU0.269931486584710.287795101799520.85658938368824
AZA vs. IL7-0.004657740075422530.9806917549190620.999311006273513
AZA vs. SAHA0.235325414027480.3361863941415710.708765553187419
DISU vs. CD30.09081953153684160.8034396516872760.865139852515591
DISU vs. IL7-0.28367302817110.2617701353633770.64744623307677
DISU vs. SAHA-0.03366783432719480.9090485990793170.978778263230015
DMSO vs. AZA-0.06134170478480650.7147811379948841
DMSO vs. CD3-0.2382088102654460.4565710928519370.568121212010774
DMSO vs. DISU-0.3327810931371910.1742912117965440.686505112852566
DMSO vs. IL70.06381354607809620.7229985485364310.943887896303078
DMSO vs. SAHA0.2892739699618610.2215055582007070.566583548479778
HIV vs. Mock in Activation0.1230634496541650.8432878247960740.999983755607037
HIV vs. Mock in Latency0.07072490527656780.6686312265431610.999834320637052
IL7 vs. CD3-0.1639626099129920.6099633467535580.725077091925582
SAHA vs. CD30.04343029638932590.9021096167044680.932491386496765
SAHA vs. IL70.2363176260440390.3336120508580410.584885079993018
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.248763 0.0652822
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.017 1.101 1.229 1.373 1.236
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1WD2 NMR - A=336-394.
2M9Y NMR - A=325-396.
4KBL X-ray 3.3Å A/B=1-557.
4KC9 X-ray 3.6Å A=1-557.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found