Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000146
UniProt IDQ14865
Primary gene name(s)ARID5B
Synonym gene name(s)DESRT, MRF2
Protein nameAT-rich interactive domain-containing protein 5B
Protein functionTranscription coactivator that binds to the 5'-AATA[CT]-3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-336, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3, H3K9me2, followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. Required for adipogenesis: regulates triglyceride metabolism in adipocytes by regulating expression of adipogenic genes. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may also act as a regulator of smooth muscle cell differentiation and proliferation. Represses the cytomegalovirus enhancer. {ECO:0000269|PubMed:21532585}.
Subcellular locationNucleus {ECO:0000255|PROSITE-ProRule:PRU00355}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q14865
Gene Ontology
(Biological Process)
Complete annatation
adipose tissue development [GO:0060612];
adrenal gland development [GO:0030325];
cell development [GO:0048468];
face morphogenesis [GO:0060325];
fat cell differentiation [GO:0045444];
fat pad development [GO:0060613];
female gonad development [GO:0008585];
fibroblast migration [GO:0010761];
kidney development [GO:0001822];
liver development [GO:0001889];
male gonad development [GO:0008584];
multicellular organism growth [GO:0035264];
muscle organ morphogenesis [GO:0048644];
negative regulation of transcription, DNA-templated [GO:0045892];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
palate development [GO:0060021];
platelet-derived growth factor receptor signaling pathway [GO:0048008];
positive regulation of sequence-specific DNA binding transcription factor activity [GO:0051091];
post-embryonic development [GO:0009791];
skeletal system morphogenesis [GO:0048705];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
DNA binding [GO:0003677];
histone demethylase activity [GO:0032452];
RNA polymerase II regulatory region sequence-specific DNA binding [GO:0000977];
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding [GO:0001227];
transcription regulatory region DNA binding [GO:0044212]
Gene Ontology
(Cellular Component)
Complete annatation
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction123918
Phylogenetic treeQ14865
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.5794629472812320.1494804544424980.242202688600014
AZA vs. DISU0.07210239336456390.7759094750689840.976444104025318
AZA vs. IL70.4458057707018290.02101411637728020.464059243345433
AZA vs. SAHA0.0438516821609510.8576762694005240.967133804138028
DISU vs. CD30.6395163710741550.1083633248247490.19852561477981
DISU vs. IL70.3650208549668020.1480343684081770.500724412892221
DISU vs. SAHA-0.02728513200655310.9254335245276050.982806795139101
DMSO vs. AZA-0.1051073807790890.532757086633441
DMSO vs. CD30.4652941396538630.2468013734822370.352189323814521
DMSO vs. DISU-0.1783429052342010.4655263522150810.895060235759284
DMSO vs. IL70.5578465281060370.002042939100206030.0883709843899598
DMSO vs. SAHA0.1411151292766080.5503706461178230.838773992076217
HIV vs. Mock in Activation-0.005726106736151130.9947936098234960.999983755607037
HIV vs. Mock in Latency-0.03039257583153540.8544249289805520.999834320637052
IL7 vs. CD31.033734955728970.01112565223423520.0325751663027637
SAHA vs. CD30.5982837317814230.1252496638383690.208726355639082
SAHA vs. IL7-0.4059021083184270.096339738207410.280858377524179
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.468161 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1IG6 NMR - A=318-424.
2OEH NMR - A=318-424.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found