Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000137
UniProt IDA7KAX9
Primary gene name(s)ARHGAP32
Synonym gene name(s)GRIT, KIAA0712, RICS
Protein nameRho GTPase-activating protein 32
Protein functionGTPase-activating protein, GAP promoting GTP hydrolysis on RHOA, CDC42 and RAC1 small GTPases. May be involved in the differentiation of neuronal cells during the formation of neurite extensions. Involved in NMDA receptor activity-dependent actin reorganization in dendritic spines. May mediate cross-talks between Ras- and Rho-regulated signaling pathways in cell growth regulation. Isoform 2 has higher GAP activity, By similarity. {ECO:0000250, ECO:0000269|PubMed:12446789, ECO:0000269|PubMed:12454018, ECO:0000269|PubMed:12531901, ECO:0000269|PubMed:12788081, ECO:0000269|PubMed:12819203, ECO:0000269|PubMed:12857875, ECO:0000269|PubMed:17663722}.
Subcellular locationCell junction, synapse, postsynaptic cell membrane, postsynaptic density {ECO:0000250|UniProtKB:Q811P8}. Cell projection, dendritic spine {ECO:0000250|UniProtKB:Q811P8}. Cytoplasm, cell cortex {ECO:0000269|PubMed:12446789}. Endosome membrane {ECO:0000269|PubMed:17663722}. Golgi apparatus membrane {ECO:0000269|PubMed:17663722}. Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q811P8}. Membrane {ECO:0000269|PubMed:17663722}. Note=Association to membrane via PX domain, PubMed:17663722. Associated with cortical actin in undifferentiated neuroblastoma cells, but localized to dendritic spine and postsynaptic density after differentiation, By similarity. Colocalizes with EGFR at the cell membrane upon EGF treatment, PubMed:12446789. Colocalizes with GAB2 at the cell membrane, PubMed:12819203. {ECO:0000250|UniProtKB:Q811P8, ECO:0000269|PubMed:12446789, ECO:0000269|PubMed:12819203, ECO:0000269|PubMed:17663722}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: A7KAX9
Gene Ontology
(Biological Process)
Complete annatation
regulation of small GTPase mediated signal transduction [GO:0051056];
small GTPase mediated signal transduction [GO:0007264]
Gene Ontology
(Molecular Function)
Complete annatation
GTPase activator activity [GO:0005096];
phosphatidylinositol binding [GO:0035091]
Gene Ontology
(Cellular Component)
Complete annatation
actin cytoskeleton [GO:0015629];
cell cortex [GO:0005938];
cell junction [GO:0030054];
cytosol [GO:0005829];
dendritic spine [GO:0043197];
endoplasmic reticulum membrane [GO:0005789];
endosome membrane [GO:0010008];
Golgi membrane [GO:0000139];
postsynaptic density [GO:0014069];
postsynaptic membrane [GO:0045211]
Protein-protein interaction115091
Phylogenetic treeA7KAX9
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.861439332482150.007317104786543240.0200006172184947
AZA vs. DISU0.1132782354349520.8417319637295820.986952988585852
AZA vs. IL7-1.099266490951420.05882170899659430.700253052677785
AZA vs. SAHA0.5086959736472640.3933992819593880.754954793974071
DISU vs. CD31.959050491346720.003194145337940710.0111584117784747
DISU vs. IL7-1.219511298720960.02725948560962940.210831181822562
DISU vs. SAHA0.3933647216270610.4879261652414890.817382218304424
DMSO vs. AZA-0.00930186962484620.9865325858427061
DMSO vs. CD31.827564783534390.00453765037478160.0126124913092407
DMSO vs. DISU-0.1281067757745470.8033190565676060.977285200264502
DMSO vs. IL7-1.080855687103280.04059505270003840.408764355963649
DMSO vs. SAHA0.5096290558188530.3530079044490880.702682241809298
HIV vs. Mock in Activation-1.15590436859780.2377716463273430.999983755607037
HIV vs. Mock in Latency-0.7558561259821810.0002143494419515330.0197540603640128
IL7 vs. CD30.7746614453038530.2557657443572340.388311549626973
SAHA vs. CD32.339191272547660.0007736210677649070.00286730681043216
SAHA vs. IL71.598285190904480.006639415632812450.0471198452208168
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander -0.84 1.47E-10 1.16E-08
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.515353 0.00241278
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3IUG X-ray 1.7Å A/B=367-577.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
HIV-1 virus replication enhanced by expression of human gene 18187620

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found