Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000129
UniProt IDP62330
Primary gene name(s)ARF6
Synonym gene name(s)unknown
Protein nameADP-ribosylation factor 6
Protein functionGTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling. Required for normal completion of mitotic cytokinesis. Plays a role in the reorganization of the actin cytoskeleton and the formation of stress fibers. May also modulate vesicle budding and uncoating within the Golgi apparatus. Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension. Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. {ECO:0000269|PubMed:11266366, ECO:0000269|PubMed:14978216, ECO:0000269|PubMed:16099990, ECO:0000269|PubMed:16737952, ECO:0000269|PubMed:18400762, ECO:0000269|PubMed:21170023, ECO:0000269|PubMed:7589240}.
Subcellular locationGolgi apparatus. Cell membrane;
Lipid-anchor. Endosome membrane;
Lipid-anchor. Recycling endosome membrane {ECO:0000305};
Lipid-anchor {ECO:0000305}. Cell projection, filopodium membrane;
Lipid-anchor. Midbody {ECO:0000250}. Cytoplasm {ECO:0000250}. Cleavage furrow {ECO:0000250}. Note=Distributed throughout the cytoplasm during metaphase. Transiently detected at the ingressing cleavage furrow during mitotic cytokinesis. Recruited to the midbody at later stages of cytokinesis;
this requires interaction with KIF23, By similarity. Recruited to the cell membrane in association with CYTH2 and ARL4C. Colocalizes with DAB2IP at the plasma membrane and endocytic vesicles. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P62330
Gene Ontology
(Biological Process)
Complete annatation
cell adhesion [GO:0007155];
cell cycle [GO:0007049];
cell division [GO:0051301];
cortical actin cytoskeleton organization [GO:0030866];
establishment of epithelial cell polarity [GO:0090162];
hepatocyte apoptotic process [GO:0097284];
liver development [GO:0001889];
movement of cell or subcellular component [GO:0006928];
myeloid cell apoptotic process [GO:0033028];
negative regulation of dendrite development [GO:2000171];
negative regulation of receptor-mediated endocytosis [GO:0048261];
positive regulation of actin filament polymerization [GO:0030838];
positive regulation of establishment of protein localization to plasma membrane [GO:0090004];
protein localization to cell surface [GO:0034394];
protein localization to endosome [GO:0036010];
protein transport [GO:0015031];
regulation of dendritic spine development [GO:0060998];
regulation of filopodium assembly [GO:0051489];
regulation of Rac protein signal transduction [GO:0035020];
ruffle organization [GO:0031529];
small GTPase mediated signal transduction [GO:0007264];
vesicle-mediated transport [GO:0016192]
Gene Ontology
(Molecular Function)
Complete annatation
GTPase activity [GO:0003924];
GTP binding [GO:0005525];
thioesterase binding [GO:0031996]
Gene Ontology
(Cellular Component)
Complete annatation
cell cortex [GO:0005938];
cleavage furrow [GO:0032154];
early endosome [GO:0005769];
endocytic vesicle [GO:0030139];
endosome [GO:0005768];
extracellular exosome [GO:0070062];
filopodium membrane [GO:0031527];
focal adhesion [GO:0005925];
Golgi apparatus [GO:0005794];
membrane [GO:0016020];
midbody [GO:0030496];
myelin sheath [GO:0043209];
plasma membrane [GO:0005886];
recycling endosome membrane [GO:0055038];
ruffle [GO:0001726]
Protein-protein interaction106877
Phylogenetic treeP62330
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.2275380151509450.4865609198988660.604521159402828
AZA vs. DISU-0.1891455711762630.4540061084470.921997150117399
AZA vs. IL70.0005555268212249010.9976899397628640.999434875292916
AZA vs. SAHA-0.3907212403794940.1093634714032720.421191867126451
DISU vs. CD3-0.4286589887877050.2369391957282730.362010996938376
DISU vs. IL70.180235312018760.4737858051441680.809878785865555
DISU vs. SAHA-0.1995208547725690.4933005205378380.820121209368188
DMSO vs. AZA-0.1008577814430680.5462675056007361
DMSO vs. CD3-0.3380978771738540.290061267608870.399759674003076
DMSO vs. DISU0.08694982241456020.7211485568601290.96344935743636
DMSO vs. IL70.1084988517708920.545488677126680.897138871287344
DMSO vs. SAHA-0.2959919008489560.2091425998593440.550565610913566
HIV vs. Mock in Activation0.1627323238192540.793441343015080.999983755607037
HIV vs. Mock in Latency0.08385744913865650.6110508446878690.999834320637052
IL7 vs. CD3-0.2206415838227420.4916988739432650.62259552477712
SAHA vs. CD3-0.6416622512616270.07010271943982070.13104043437833
SAHA vs. IL7-0.3938309091245430.1058253274931140.29706949555329
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.209096 0.473144
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.065 0.957 0.981 1.048 1.045
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category