Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000110
UniProt IDQ9BTT0
Primary gene name(s)ANP32E
Synonym gene name(s)unknown
Protein nameAcidic leucine-rich nuclear phosphoprotein 32 family member E
Protein functionHistone chaperone that specifically mediates the genome-wide removal of histone H2A.Z/H2AFZ from the nucleosome: removes H2A.Z/H2AFZ from its normal sites of deposition, especially from enhancer and insulator regions. Not involved in deposition of H2A.Z/H2AFZ in the nucleosome. May stabilize the evicted H2A.Z/H2AFZ-H2B dimer, thus shifting the equilibrium towards dissociation and the off-chromatin state, PubMed:24463511. Inhibits activity of protein phosphatase 2A, PP2A. Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis. {ECO:0000269|PubMed:24463511}.
Subcellular locationCytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9BTT0
Gene Ontology
(Biological Process)
Complete annatation
covalent chromatin modification [GO:0016569];
histone exchange [GO:0043486];
nucleocytoplasmic transport [GO:0006913];
nucleosome assembly [GO:0006334];
regulation of apoptotic process [GO:0042981]
Gene Ontology
(Molecular Function)
Complete annatation
histone binding [GO:0042393];
phosphatase inhibitor activity [GO:0019212]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasmic, membrane-bounded vesicle [GO:0016023];
nucleus [GO:0005634];
Swr1 complex [GO:0000812]
Protein-protein interaction123548
Phylogenetic treeQ9BTT0
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.654960229425638.17693456722779e-076.45505203039858e-06
AZA vs. DISU-0.09663179741015680.7026778099655220.970579553778938
AZA vs. IL70.2171490657281760.2582460172702530.999311006273513
AZA vs. SAHA-0.02297778122135210.9248632172549910.982479497044848
DISU vs. CD3-1.764067460327592.41853813243154e-062.11143213612639e-05
DISU vs. IL70.3042574771791140.2283891126491170.612082821899632
DISU vs. SAHA0.07470155734864760.7977313758399970.946755089663451
DMSO vs. AZA-0.1202021627226110.4726325600522491
DMSO vs. CD3-1.781937919433196.20927408379046e-085.66582156706864e-07
DMSO vs. DISU-0.02433588169070510.9205406112351980.988899823982894
DMSO vs. IL70.3439359652265790.05582967716514710.463763012974044
DMSO vs. SAHA0.08895100634639870.7056221525283260.911755337604151
HIV vs. Mock in Activation0.2091376741897810.7366241733453720.999983755607037
HIV vs. Mock in Latency0.2742932113624870.09690657322066040.999834320637052
IL7 vs. CD3-1.430361599746191.21722424555326e-059.17015554147043e-05
SAHA vs. CD3-1.702779398901692.67757129268631e-061.96259724614702e-05
SAHA vs. IL7-0.2435173261641530.317077295350560.565833781038688
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
-0.3842 0.03339

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
2 0.000151729 1.2 0.847514237 1.3 0.525278614
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.29217 0.0380012
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.052 0.988 0.965 0.864 0.983
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4CAY X-ray 1.4Å C=215-240.
4NFT X-ray 2.6Å E/F=185-232.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Rev interacts with 22174317
retropepsin cleaves 22944692

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found
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