Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000093
UniProt IDQ9H1A4
Primary gene name(s)ANAPC1
Synonym gene name(s)TSG24
Protein nameAnaphase-promoting complex subunit 1
Protein functionComponent of the anaphase promoting complex/cyclosome, APC/C, a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. {ECO:0000269|PubMed:18485873}.
Subcellular locationunknown
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9H1A4
Gene Ontology
(Biological Process)
Complete annatation
anaphase-promoting complex-dependent catabolic process [GO:0031145];
cell division [GO:0051301];
mitotic nuclear division [GO:0007067];
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [GO:0051436];
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition [GO:0051437];
proteasome-mediated ubiquitin-dependent protein catabolic process [GO:0043161];
protein K11-linked ubiquitination [GO:0070979];
protein ubiquitination involved in ubiquitin-dependent protein catabolic process [GO:0042787];
regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [GO:0051439]
Gene Ontology
(Molecular Function)
Complete annatation
unknown
Gene Ontology
(Cellular Component)
Complete annatation
anaphase-promoting complex [GO:0005680];
cytosol [GO:0005829];
nucleoplasm [GO:0005654]
Protein-protein interaction122229
Phylogenetic treeQ9H1A4
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.122244296129610.0007001054523764210.00265458852282199
AZA vs. DISU-0.07503543066421270.7672105182777080.976021571236163
AZA vs. IL70.3496521385708480.06944560684463050.746614573444448
AZA vs. SAHA-0.01280088568506830.9581635519533210.990779256780515
DISU vs. CD3-1.209678385181010.00102216169229030.00423414821368069
DISU vs. IL70.4158947544808750.09969477986779660.418634765043742
DISU vs. SAHA0.06336787738056920.8281037536583650.955481976816337
DMSO vs. AZA0.06896257117554970.6811801282894411
DMSO vs. CD3-1.064829174902310.0009791771002678470.00336097129540559
DMSO vs. DISU0.1421356313654030.56077897619680.925732173460781
DMSO vs. IL70.2880362608112350.1096124976368470.594819439120037
DMSO vs. SAHA-0.08857784352143890.7071874531499460.911894760281734
HIV vs. Mock in Activation0.0669081302701960.9144336353264510.999983755607037
HIV vs. Mock in Latency-0.007087370203915040.9658237414665730.999834320637052
IL7 vs. CD3-0.7646045058896810.0178475177397470.0477910170352117
SAHA vs. CD3-1.159844149181940.001176459865759980.00411446975562844
SAHA vs. IL7-0.3661852052912280.1330826858963240.341165617191232
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.418803 0.0102346
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.921 1.017 0.938 0.939 1.103
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4UI9 EM 3.6Å A=1-1944.
5A31 EM 4.3Å A=11-1897.
5G04 EM 4.0Å A=1-1943.
5G05 EM 3.4Å A=1-1944.
5KHR EM 6.1Å A=1-1944.
5KHU EM 4.8Å A=1-1944.
5L9U EM 6.4Å A=1-1944.
5LCW EM 4.0Å A=1-1944.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
retropepsin interacts with 22190034

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04110 Cell cycle - Homo sapiens (human)
hsa04114 Oocyte meiosis - Homo sapiens (human)
hsa04120 Ubiquitin mediated proteolysis - Homo sapiens (human)
hsa04914 Progesterone-mediated oocyte maturation - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)
Menu