Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000076
UniProt IDQ99996
Primary gene name(s)AKAP9
Synonym gene name(s)AKAP350, AKAP450, KIAA0803
Protein nameA-kinase anchor protein 9
Protein functionScaffolding protein that assembles several protein kinases and phosphatases on the centrosome and Golgi apparatus. Required to maintain the integrity of the Golgi apparatus, PubMed:10202149, PubMed:15047863. Recruited to the Golgi apparatus by GM130/GOLGA2 and is required for microtubule nucleation at the cis-side of the Golgi apparatusGM130/GOLGA2. {ECO:0000269|PubMed:10202149, ECO:0000269|PubMed:15047863, ECO:0000269|PubMed:19242490}.; FUNCTION: Isoform 4: Associated with the N-methyl-D-aspartate receptor and is specifically found in the neuromuscular junction, NMJ as well as in neuronal synapses, suggesting a role in the organization of postsynaptic specializations. {ECO:0000269|PubMed:9482789}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:9915845}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:12270714, ECO:0000269|PubMed:9915845}. Golgi apparatus {ECO:0000269|PubMed:15047863, ECO:0000269|PubMed:19242490}. Note=Cytoplasmic in parietal cells. {ECO:0000269|PubMed:9915845}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q99996
Gene Ontology
(Biological Process)
Complete annatation
cardiac conduction [GO:0061337];
cellular response to cAMP [GO:0071320];
chemical synaptic transmission [GO:0007268];
G2/M transition of mitotic cell cycle [GO:0000086];
MAPK cascade [GO:0000165];
microtubule nucleation [GO:0007020];
negative regulation of adenylate cyclase activity [GO:0007194];
positive regulation of peptidyl-serine phosphorylation [GO:0033138];
positive regulation of potassium ion transmembrane transporter activity [GO:1901018];
regulation of cardiac muscle cell action potential involved in regulation of contraction [GO:0098909];
regulation of heart rate by cardiac conduction [GO:0086091];
regulation of membrane repolarization [GO:0060306];
regulation of ventricular cardiac muscle cell membrane repolarization [GO:0060307];
response to electrical stimulus [GO:0051602];
signal transduction [GO:0007165];
transport [GO:0006810]
Gene Ontology
(Molecular Function)
Complete annatation
ion channel binding [GO:0044325];
potassium channel regulator activity [GO:0015459];
protein complex scaffold [GO:0032947];
protein kinase A regulatory subunit binding [GO:0034237];
Ras guanyl-nucleotide exchange factor activity [GO:0005088];
receptor binding [GO:0005102]
Gene Ontology
(Cellular Component)
Complete annatation
centrosome [GO:0005813];
cis-Golgi network [GO:0005801];
cytoskeleton [GO:0005856];
cytosol [GO:0005829];
Golgi apparatus [GO:0005794];
Golgi stack [GO:0005795];
intracellular membrane-bounded organelle [GO:0043231];
voltage-gated potassium channel complex [GO:0008076]
Protein-protein interaction115445
Phylogenetic treeQ99996
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.6022879636471360.06647121634387990.125987697778541
AZA vs. DISU0.3395225475152960.1818381136239890.76836786777268
AZA vs. IL7-0.01111773670297110.9562444642765690.999311006273513
AZA vs. SAHA-0.04994114402442650.8376140566496590.961440456451378
DISU vs. CD30.9285300840260180.01154846272358350.0326668281282224
DISU vs. IL7-0.3589600439615240.1566463576930720.513134255154945
DISU vs. SAHA-0.3894792813521660.1854360384200040.551686629966569
DMSO vs. AZA-0.06191141114408390.7111922549833351
DMSO vs. CD30.5296970364211580.09809020383285590.168325426670689
DMSO vs. DISU-0.4030348004316940.1008240489069080.572469160742217
DMSO vs. IL70.05790656094263820.7470113083911580.950434477375902
DMSO vs. SAHA0.003367931872081990.9885877008668460.996342259020082
HIV vs. Mock in Activation0.1852421189371170.7690480877369530.999983755607037
HIV vs. Mock in Latency0.1177338580883980.4749250754308610.999834320637052
IL7 vs. CD30.6005616491056610.06213349946255560.130764903295043
SAHA vs. CD30.5256720438777080.1373276210126540.224436313703579
SAHA vs. IL7-0.04420441314467220.855794554854590.939703315841935
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.101519 0.607446
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.944 1.141 0.935 0.884 1.253
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
retropepsin interacts with 22190034

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found