Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000075
UniProt IDQ9ULX6
Primary gene name(s)AKAP8L
Synonym gene name(s)NAKAP, NAKAP95
Protein nameA-kinase anchor protein 8-like
Protein functionCould play a role in constitutive transport element, CTE-mediated gene expression by association with DHX9. Increases CTE-dependent nuclear unspliced mRNA export, PubMed:10748171, PubMed:11402034. Proposed to target PRKACA to the nucleus but does not seem to be implicated in the binding of regulatory subunit II of PKA, PubMed:10761695, PubMed:11884601. May be involved in nuclear envelope breakdown and chromatin condensation. May be involved in anchoring nuclear membranes to chromatin in interphase and in releasing membranes from chromating at mitosis, PubMed:11034899. May regulate the initiation phase of DNA replication when associated with TMPO isoform Beta, PubMed:12538639. Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function seems to act redundantly with AKAP8, PubMed:16980585. May be involved in regulation of pre-mRNA splicing, PubMed:17594903. {ECO:0000269|PubMed:10748171, ECO:0000269|PubMed:11034899, ECO:0000269|PubMed:11402034, ECO:0000269|PubMed:11884601, ECO:0000269|PubMed:12538639, ECO:0000269|PubMed:16980585, ECO:0000305|PubMed:10761695}.; FUNCTION:, Microbial infection In case of EBV infection, may target PRKACA to EBNA-LP-containing nuclear sites to modulate transcription from specific promoters, PubMed:11884601. Can synergize with DHX9 to activate the CTE-mediated gene expression of type D retroviruses, PubMed:11402034. In case of HIV-1 infection, involved in the DHX9-promoted annealing of host tRNA(Lys3 to viral genomic RNA as a primer in reverse transcription; in vitro negatively regulates DHX9 annealing activity, PubMed:25034436. {ECO:0000269|PubMed:11402034, ECO:0000269|PubMed:11884601, ECO:0000269|PubMed:25034436}.
Subcellular locationNucleus {ECO:0000269|PubMed:10748171, ECO:0000269|PubMed:10761695}. Nucleus matrix {ECO:0000269|PubMed:11034899, ECO:0000269|PubMed:16391387}. Nucleus speckle {ECO:0000269|PubMed:17594903}. Nucleus, PML body {ECO:0000269|PubMed:11884601}. Cytoplasm {ECO:0000269|PubMed:10748171}. Note=Colocalizes with PRPF40A in the nuclear matrix, PubMed:16391387. Nuclear at steady state but shuttles between the nucleus and cytoplasm, PubMed:10748171. The shuttling property has been questioned, PubMed:11034899. Colocalizes with EBNA-LP in PML bodies, PubMed:11884601. {ECO:0000269|PubMed:10748171, ECO:0000269|PubMed:11034899, ECO:0000269|PubMed:11884601, ECO:0000269|PubMed:16391387}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9ULX6
Gene Ontology
(Biological Process)
Complete annatation
cell cycle G2/M phase transition [GO:0044839];
mitotic chromosome condensation [GO:0007076];
mRNA processing [GO:0006397];
nuclear envelope disassembly [GO:0051081];
positive regulation of histone deacetylation [GO:0031065];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
regulation of histone phosphorylation [GO:0033127];
regulation of mRNA export from nucleus [GO:0010793];
RNA splicing [GO:0008380];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
DEAD/H-box RNA helicase binding [GO:0017151];
DNA binding [GO:0003677];
histone deacetylase binding [GO:0042826];
lamin binding [GO:0005521];
metal ion binding [GO:0046872];
poly(A RNA binding [GO:0044822]
Gene Ontology
(Cellular Component)
Complete annatation
chromatin [GO:0000785];
cytoplasm [GO:0005737];
nuclear matrix [GO:0016363];
nuclear speck [GO:0016607];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
PML body [GO:0016605]
Protein-protein interaction117940
Phylogenetic treeQ9ULX6
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.6085562343741320.06533692855884870.124181360307562
AZA vs. DISU0.1226846435281840.6279117869795730.960057478314726
AZA vs. IL7-0.2919149687736240.130643168111840.897328354403451
AZA vs. SAHA0.4020812024505830.1007357926663330.400951749838685
DISU vs. CD30.718165555144640.04976817513076090.107457531277574
DISU vs. IL7-0.4239824841545070.09334353260679840.406152341835042
DISU vs. SAHA0.2816259320454270.3351107615651760.714633741719471
DMSO vs. AZA0.05594028426669560.7393836767451481
DMSO vs. CD30.6519893229025240.04335660847141450.0853144339892712
DMSO vs. DISU-0.06884161056592550.7779249806334930.971585548411322
DMSO vs. IL7-0.3403920910573230.05957861316536030.470727250245181
DMSO vs. SAHA0.3403249939906120.1504178806490970.46750832733812
HIV vs. Mock in Activation0.2380925759266320.7029125919449840.999983755607037
HIV vs. Mock in Latency-0.01061309651604020.9488234595315370.999834320637052
IL7 vs. CD30.3227655245242090.3171240286415210.455239093687013
SAHA vs. CD30.9865690796453120.005673042056246410.0158908445701921
SAHA vs. IL70.6908106265691140.00481863424400120.037402725331655
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0131515 0.961335
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.87 0.701 0.727 0.76 0.737
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
reverse transcriptase associates with 25034436
Vif interacts with 22190034
Pol incorporates 25034436

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa05169 Epstein-Barr virus infection - Homo sapiens (human)