Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000068
UniProt IDQ8N157
Primary gene name(s)AHI1
Synonym gene name(s)unknown
Protein nameJouberin
Protein functionInvolved in vesicle trafficking and required for ciliogenesis, formation of primary non-motile cilium, and recruitment of RAB8A to the basal body of primary cilium. Component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Involved in neuronal differentiation. {ECO:0000250|UniProtKB:Q8K3E5}.
Subcellular locationCytoplasm, cytoskeleton, cilium basal body {ECO:0000269|PubMed:23532844}. Cell junction, adherens junction {ECO:0000269|PubMed:18633336, ECO:0000269|PubMed:23532844}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole {ECO:0000250|UniProtKB:Q8K3E5}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8N157
Gene Ontology
(Biological Process)
Complete annatation
cellular protein localization [GO:0034613];
central nervous system development [GO:0007417];
cilium assembly [GO:0060271];
cloaca development [GO:0035844];
heart looping [GO:0001947];
hindbrain development [GO:0030902];
Kupffer's vesicle development [GO:0070121];
left/right axis specification [GO:0070986];
morphogenesis of a polarized epithelium [GO:0001738];
negative regulation of apoptotic process [GO:0043066];
otic vesicle development [GO:0071599];
photoreceptor cell outer segment organization [GO:0035845];
positive regulation of polarized epithelial cell differentiation [GO:0030862];
positive regulation of receptor internalization [GO:0002092];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
pronephric duct morphogenesis [GO:0039023];
pronephric nephron tubule morphogenesis [GO:0039008];
regulation of behavior [GO:0050795];
retina layer formation [GO:0010842];
specification of axis polarity [GO:0065001];
transmembrane receptor protein tyrosine kinase signaling pathway [GO:0007169];
vesicle-mediated transport [GO:0016192]
Gene Ontology
(Molecular Function)
Complete annatation
identical protein binding [GO:0042802]
Gene Ontology
(Cellular Component)
Complete annatation
adherens junction [GO:0005912];
cell-cell junction [GO:0005911];
centriole [GO:0005814];
centrosome [GO:0005813];
ciliary basal body [GO:0036064];
cilium [GO:0005929];
cytosol [GO:0005829];
nonmotile primary cilium [GO:0031513];
primary cilium [GO:0072372];
TCTN-B9D complex [GO:0036038]
Protein-protein interaction120163
Phylogenetic treeQ8N157
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD34.3097589902882400
AZA vs. DISU0.3045745119791750.2346298721863080.819503318633774
AZA vs. IL7-0.0185207892303290.9244360596764230.999311006273513
AZA vs. SAHA0.5483179254761180.02670240173936760.185489402681671
DISU vs. CD3-4.0187174166106700
DISU vs. IL7-0.3318401777215060.1919136877368990.565610721922531
DISU vs. SAHA0.2452079951491180.4114897268340970.767900157641986
DMSO vs. AZA-0.01419435799910450.9338522886808991
DMSO vs. CD3-4.3336540994015300
DMSO vs. DISU-0.3200424015353390.1965422843264270.713677194394938
DMSO vs. IL70.002456968434454750.9892839101318020.998557411800262
DMSO vs. SAHA0.5552428580364680.01998195224485940.141450447290877
HIV vs. Mock in Activation0.1974898924174620.7508720787726720.999983755607037
HIV vs. Mock in Latency0.3495619591333880.1881343059041760.999834320637052
IL7 vs. CD3-4.3205909108167900
SAHA vs. CD3-3.7861701434002600
SAHA vs. IL70.5633825165039220.0231557545688580.110423867248012
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock 0.839 4.46E-07 1.36E-05
Infected vs. Bystander 0.88 2.03E-07 4.66E-06
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.020674 0.93852
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4ESR X-ray 1.5Å A/B=1048-1116.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found