Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000066
UniProt IDP23526
Primary gene name(s)AHCY
Synonym gene name(s)SAHH
Protein nameAdenosylhomocysteinase
Protein functionAdenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. {ECO:0000269|PubMed:12590576}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:17081065}. Melanosome {ECO:0000269|PubMed:17081065}. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P23526
Gene Ontology
(Biological Process)
Complete annatation
chronic inflammatory response to antigenic stimulus [GO:0002439];
circadian sleep/wake cycle [GO:0042745];
homocysteine biosynthetic process [GO:0071268];
methylation [GO:0032259];
one-carbon metabolic process [GO:0006730];
response to hypoxia [GO:0001666];
response to nutrient [GO:0007584];
S-adenosylhomocysteine catabolic process [GO:0019510];
S-adenosylmethionine cycle [GO:0033353];
sulfur amino acid metabolic process [GO:0000096]
Gene Ontology
(Molecular Function)
Complete annatation
adenosylhomocysteinase activity [GO:0004013];
adenyl nucleotide binding [GO:0030554];
NAD binding [GO:0051287]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
melanosome [GO:0042470];
neuron projection [GO:0043005];
nucleus [GO:0005634]
Protein-protein interaction106696
Phylogenetic treeP23526
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.935232704047751.18113409053322e-081.37249617789067e-07
AZA vs. DISU0.1058934264675460.6772737343834350.964884761087644
AZA vs. IL70.4515456930198890.02011349414700950.458244372746071
AZA vs. SAHA-0.4769513956792690.05443370923899960.284385890813731
DISU vs. CD3-1.84181677630518.25566766171626e-078.1818497260165e-06
DISU vs. IL70.3370300298686430.1822872807004620.552691222155967
DISU vs. SAHA-0.581710010826850.04762408772450440.272078326799093
DMSO vs. AZA-0.0566540865907040.7633660927887481
DMSO vs. CD3-2.00833092311921.43451528433758e-091.80241641022065e-08
DMSO vs. DISU-0.1659413563764450.4984973559498750.907526896115356
DMSO vs. IL70.5162551950488590.004481710174850.135123561771727
DMSO vs. SAHA-0.4256577643787910.07482006725817480.320193482951959
HIV vs. Mock in Activation-0.1463003634039940.8139665560348050.999983755607037
HIV vs. Mock in Latency-0.1773953903606980.2890989115369070.999834320637052
IL7 vs. CD3-1.475120024398546.73204277423789e-065.4379464488793e-05
SAHA vs. CD3-2.437140517480356.16562356725581e-111.39304268603516e-09
SAHA vs. IL7-0.9326745892742320.0001580520871731220.00280621573857201
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
-0.4813 0.02239

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.425813 0.00132191
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.044 0.926 0.862 0.826 0.824
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB02325 Isopropyl Alcohol approved unknown unknown
DB03216 (1&,39;r,2&,39;s)-9-(2-Hydroxy-3&,39;-Keto-Cyclopenten-1-Yl)Adenine experimental unknown unknown
DB03273 3&,39;-Oxo-Adenosine experimental unknown unknown
DB03769 D-Eritadenine experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1A7A X-ray 2.8Å A/B=1-432.
1LI4 X-ray 2.0Å A=1-432.
3NJ4 X-ray 2.5Å A/B/C/D=1-432.
4PFJ X-ray 2.3Å A/B=1-432.
4PGF X-ray 2.5Å A/B=1-432.
4YVF X-ray 2.7Å A/B=1-432.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
retropepsin interacts with 22190034
Tat interacts with 22190034

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00270 Cysteine and methionine metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)