Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000060
UniProt IDP52594
Primary gene name(s)AGFG1
Synonym gene name(s)HRB, RAB, RIP
Protein nameArf-GAP domain and FG repeat-containing protein 1
Protein functionRequired for vesicle docking or fusion during acrosome biogenesis, By similarity. May play a role in RNA trafficking or localization. In case of infection by HIV-1, acts as a cofactor for viral Rev and promotes movement of Rev-responsive element-containing RNAs from the nuclear periphery to the cytoplasm. This step is essential for HIV-1 replication. {ECO:0000250, ECO:0000269|PubMed:10613896, ECO:0000269|PubMed:14701878, ECO:0000269|PubMed:15749819}.
Subcellular locationNucleus {ECO:0000269|PubMed:10613896, ECO:0000269|PubMed:7634337}. Cytoplasmic vesicle {ECO:0000269|PubMed:10613896}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P52594
Gene Ontology
(Biological Process)
Complete annatation
acrosome assembly [GO:0001675];
intermediate filament organization [GO:0045109];
mRNA export from nucleus [GO:0006406];
multicellular organism development [GO:0007275];
spermatid nucleus differentiation [GO:0007289]
Gene Ontology
(Molecular Function)
Complete annatation
DNA binding [GO:0003677];
GTPase activator activity [GO:0005096];
metal ion binding [GO:0046872];
RNA binding [GO:0003723]
Gene Ontology
(Cellular Component)
Complete annatation
cell projection [GO:0042995];
cytoplasmic, membrane-bounded vesicle [GO:0016023];
intracellular membrane-bounded organelle [GO:0043231];
neuronal cell body [GO:0043025];
nuclear pore [GO:0005643]
Protein-protein interaction109503
Phylogenetic treeP52594
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.966100376968236.60962629073225e-098.08606229333738e-08
AZA vs. DISU0.1718535651430270.4979949701905830.934754900374469
AZA vs. IL70.3582370476705460.06337125287601790.723909865561374
AZA vs. SAHA0.3613653714716410.1399631009865920.47518103249367
DISU vs. CD3-1.806904357673461.37399245447867e-061.27516925078883e-05
DISU vs. IL70.1774926375340750.4815352841009630.815232336082343
DISU vs. SAHA0.1909601251490650.5148157771125690.83400930633812
DMSO vs. AZA-0.03952415954651030.814364058475561
DMSO vs. CD3-2.016979832915681.19112930629939e-091.52116612893509e-08
DMSO vs. DISU-0.2131902776030850.3833048748226470.857979524618098
DMSO vs. IL70.40505518581950.02484961545189370.321809580752529
DMSO vs. SAHA0.3943719580094980.09582999218394260.363700280081642
HIV vs. Mock in Activation-0.06316290072498430.9190830449042940.999983755607037
HIV vs. Mock in Latency-0.01590677814477510.923596484947510.999834320637052
IL7 vs. CD3-1.600146772646641.15332881156505e-061.11111456524976e-05
SAHA vs. CD3-1.629675737745586.65765008445174e-064.41010651029438e-05
SAHA vs. IL77.42123334917512e-050.9997584234421570.99982475120859
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.126545 0.433556
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1 0.945 0.935 0.981 1.015
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2D9L NMR - A=14-134.
2OLM X-ray 1.4Å A=4-141.
2VX8 X-ray 2.2Å A/B/C/D=136-175.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa05164 Influenza A - Homo sapiens (human)