Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000056
UniProt IDQ9Y4W6
Primary gene name(s)AFG3L2
Synonym gene name(s)unknown
Protein nameAFG3-like protein 2
Protein functionATP-dependent protease which is essential for axonal and neuron development. In neurons, mediates degradation of SMDT1/EMRE before its assembly with the uniporter complex, limiting the availability of SMDT1/EMRE for MCU assembly and promoting efficient assembly of gatekeeper subunits with MCU. {ECO:0000269|PubMed:27642048}.
Subcellular locationMitochondrion membrane {ECO:0000269|PubMed:10395799};
Multi-pass membrane protein {ECO:0000269|PubMed:10395799}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9Y4W6
Gene Ontology
(Biological Process)
Complete annatation
axonogenesis [GO:0007409];
calcium ion transmembrane import into mitochondrion [GO:0036444];
cristae formation [GO:0042407];
mitochondrial calcium ion homeostasis [GO:0051560];
mitochondrial fusion [GO:0008053];
mitochondrial protein processing [GO:0034982];
muscle fiber development [GO:0048747];
myelination [GO:0042552];
nerve development [GO:0021675];
neuromuscular junction development [GO:0007528];
protein complex assembly [GO:0006461];
protein import into mitochondrial intermembrane space [GO:0045041];
proteolysis [GO:0006508];
regulation of multicellular organism growth [GO:0040014];
righting reflex [GO:0060013]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
ATP-dependent peptidase activity [GO:0004176];
metalloendopeptidase activity [GO:0004222];
metallopeptidase activity [GO:0008237];
unfolded protein binding [GO:0051082];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
integral component of membrane [GO:0016021];
mitochondrial inner membrane [GO:0005743];
mitochondrion [GO:0005739]
Protein-protein interaction116139
Phylogenetic treeQ9Y4W6
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.372414975854213.77473449002519e-050.000204532300758919
AZA vs. DISU-0.1745752531443760.490774585406090.932344706809165
AZA vs. IL70.2360224754013570.2210321442103290.989265982093417
AZA vs. SAHA-0.4096265030869430.09448282283256220.387518742753145
DISU vs. CD3-1.559418782019862.55531465502834e-050.000169836036639758
DISU vs. IL70.401770164198650.1116075506382610.437365583564967
DISU vs. SAHA-0.233793127885480.4232049245782410.775918759105463
DMSO vs. AZA-0.05854852832903310.7280269775319481
DMSO vs. CD3-1.443072967278929.47555088115504e-065.49246533376686e-05
DMSO vs. DISU0.1139402236960770.6410130297576610.9472626202031
DMSO vs. IL70.301974940443170.09426641210171770.56380531769202
DMSO vs. SAHA-0.3578013057093940.1304408639368040.432440444432813
HIV vs. Mock in Activation-0.08280541340185220.8940217363943960.999983755607037
HIV vs. Mock in Latency-0.03423809265801620.8364650467762470.999834320637052
IL7 vs. CD3-1.128178256953260.0005152990336776410.00240393139418851
SAHA vs. CD3-1.807108781360236.82182042077706e-075.78856045839585e-06
SAHA vs. IL7-0.649083238002220.008041669662576690.054091980586203
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.298446 0.170463
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.03 0.935 0.946 0.942 0.861
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00171 Adenosine triphosphate approved, nutraceutical unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2LNA NMR - A=164-251.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
HIV-1 virus replication enhanced by expression of human gene 18854154

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found