Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000055
UniProt IDQ8WTP8
Primary gene name(s)AEN
Synonym gene name(s)ISG20L1
Protein nameApoptosis-enhancing nuclease
Protein functionExonuclease with activity against single- and double-stranded DNA and RNA. Mediates p53-induced apoptosis. When induced by p53 following DNA damage, digests double-stranded DNA to form single-stranded DNA and amplifies DNA damage signals, leading to enhancement of apoptosis. {ECO:0000269|PubMed:16171785, ECO:0000269|PubMed:18264133}.
Subcellular locationNucleus. Nucleus, nucleolus. Note=Localized predomintly in the nucleolus. Translocates from the nucleolus to the nucleoplasm upon apoptosis induction.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8WTP8
Gene Ontology
(Biological Process)
Complete annatation
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [GO:0042771];
response to ionizing radiation [GO:0010212]
Gene Ontology
(Molecular Function)
Complete annatation
exonuclease activity [GO:0004527];
nucleic acid binding [GO:0003676]
Gene Ontology
(Cellular Component)
Complete annatation
nuclear membrane [GO:0031965];
nucleolus [GO:0005730];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction122292
Phylogenetic treeQ8WTP8
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.03283916171561240.9200632664971920.948962810692239
AZA vs. DISU-0.3021253162378790.233821616627750.819157190934184
AZA vs. IL70.02508655278982920.8961631423977320.999311006273513
AZA vs. SAHA-1.019191125713933.82760160693163e-050.00183970170517024
DISU vs. CD3-0.348195081544360.339806149538640.473154964203784
DISU vs. IL70.3181881753104790.2073099967590090.586127475416937
DISU vs. SAHA-0.7150042477284060.01463422386613870.133103432641265
DMSO vs. AZA-0.2456574567522570.1428966210221051
DMSO vs. CD3-0.2938551071290540.3582524273291890.472308814011922
DMSO vs. DISU0.05362242696769690.826494742077120.980747352324641
DMSO vs. IL70.2784683029239940.1218188251674460.613981541089683
DMSO vs. SAHA-0.7783897416714230.001089218788595910.0189775911710162
HIV vs. Mock in Activation0.136073223153370.826742718428090.999983755607037
HIV vs. Mock in Latency-0.3347080702730680.08000536014355060.999834320637052
IL7 vs. CD3-0.0008278016749422130.9979462236866530.998807664649995
SAHA vs. CD3-1.076103110259650.00277375594038320.00857798112488711
SAHA vs. IL7-1.046778465081522.19634013675085e-050.000581856436228161
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
0.5577 0.01181

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock 0.791 1.77E-11 4.24E-09