Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000046
UniProt IDQ9BV57
Primary gene name(s)ADI1
Synonym gene name(s)MTCBP1
Protein name1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
Protein functionCatalyzes the formation of formate and 2-keto-4-methylthiobutyrate, KMTB from 1,2-dihydroxy-3-keto-5-methylthiopentene, DHK-MTPene. Also down-regulates cell migration mediated by MMP14. Necessary for hepatitis C virus replication in an otherwise non-permissive cell line. {ECO:0000255|HAMAP-Rule:MF_03154, ECO:0000269|PubMed:11602742, ECO:0000269|PubMed:15938715}.
Subcellular locationCytoplasm. Nucleus. Cell membrane;
Peripheral membrane protein;
Cytoplasmic side. Note=Localizes to the plasma membrane when complexed to MMP14.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9BV57
Gene Ontology
(Biological Process)
Complete annatation
L-methionine biosynthetic process from methylthioadenosine [GO:0019509]
Gene Ontology
(Molecular Function)
Complete annatation
acireductone dioxygenase [iron(II-requiring] activity [GO:0010309];
metal ion binding [GO:0046872];
oxidoreductase activity [GO:0016491]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
nucleus [GO:0005634];
plasma membrane [GO:0005886]
Protein-protein interaction120547
Phylogenetic treeQ9BV57
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.8896100659484730.01787752751946660.0424531423937924
AZA vs. DISU-0.1748258915382920.4913612760600450.932344706809165
AZA vs. IL7-0.1361336808364280.4800824151050340.999311006273513
AZA vs. SAHA-2.180077017260422.97991654685248e-085.4779587838115e-06
DISU vs. CD30.7033372214388560.05854645612208460.122763552679724
DISU vs. IL70.02922324543861340.9079523908656690.982189124739072
DISU vs. SAHA-2.004377104187913.31016523080763e-073.86724807701564e-05
DMSO vs. AZA-0.1072722286613360.5235219090951641
DMSO vs. CD30.7728407042365490.04335650368699050.0853144339892712
DMSO vs. DISU0.06624156473363510.7868264170799810.973311777316403
DMSO vs. IL7-0.02183848094858140.903610930631640.979523619218739
DMSO vs. SAHA-2.079600378450421.52126142127784e-071.76361006769834e-05
HIV vs. Mock in Activation0.080577497373830.9235168065278390.999983755607037
HIV vs. Mock in Latency0.07279556447610460.6600556525747710.999834320637052
IL7 vs. CD30.7604366721133430.0402383707075520.0919376931446007
SAHA vs. CD3-1.315314771076210.009377038491373720.0242887538784809
SAHA vs. IL7-2.047435236915579.85418900922852e-086.43039156385761e-06
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.489242 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.022 0.82 0.674 0.605 0.816
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB02325 Isopropyl Alcohol approved unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4QGN X-ray 3.0Å A=1-179.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00270 Cysteine and methionine metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)