Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000045
UniProt IDP11766
Primary gene name(s)ADH5
Synonym gene name(s)ADHX, FDH
Protein nameAlcohol dehydrogenase class-3
Protein functionClass-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl glutathione.
Subcellular locationCytoplasm.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P11766
Gene Ontology
(Biological Process)
Complete annatation
ethanol oxidation [GO:0006069];
formaldehyde catabolic process [GO:0046294];
peptidyl-cysteine S-nitrosylation [GO:0018119];
positive regulation of blood pressure [GO:0045777];
respiratory system process [GO:0003016];
response to lipopolysaccharide [GO:0032496];
response to nitrosative stress [GO:0051409];
response to redox state [GO:0051775];
retinoid metabolic process [GO:0001523]
Gene Ontology
(Molecular Function)
Complete annatation
alcohol dehydrogenase, NAD activity [GO:0004022];
electron carrier activity [GO:0009055];
fatty acid binding [GO:0005504];
formaldehyde dehydrogenase activity [GO:0018467];
S-(hydroxymethylglutathione dehydrogenase activity [GO:0051903];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
mitochondrion [GO:0005739]
Protein-protein interaction106640
Phylogenetic treeP11766
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.5001904743091070.1315677216389640.219138987793399
AZA vs. DISU-0.1917152618896330.4489691739880790.918679750320323
AZA vs. IL70.04155091332862980.8288091926407990.999311006273513
AZA vs. SAHA-0.3399361991177370.1637838739591940.516609775279532
DISU vs. CD30.2963276964943590.4230551564809990.55537796186486
DISU vs. IL70.2238582544426370.3748944616783620.74699491211658
DISU vs. SAHA-0.1468884510693820.6146659461484060.878760590372236
DMSO vs. AZA-0.03699086482368320.8252181921665471
DMSO vs. CD30.4519760512236020.1631965077529140.253773264791252
DMSO vs. DISU0.1528283218921460.5312695336245770.918454079411213
DMSO vs. IL70.08577921119509790.6332177087155930.920605358172202
DMSO vs. SAHA-0.3093080683301120.1897701516984720.524926013250459
HIV vs. Mock in Activation-0.1147438056762150.8554431531055940.999983755607037
HIV vs. Mock in Latency0.0607298257219040.7130349868794320.999834320637052
IL7 vs. CD30.5492502281039310.09101393371246710.176102142474689
SAHA vs. CD30.1361727364157980.7027388829410460.782725422462791
SAHA vs. IL7-0.3845161820023870.1146582322418940.311959754202148
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.266916 0.044554
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.078 1.01 1.137 1.237 1.088
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
208847_s_at 1.4 No upregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00157 NADH nutraceutical unknown unknown
DB03704 12-Hydroxydodecanoic Acid experimental unknown unknown
DB04153 S-Hydroxymethyl Glutathione experimental unknown unknown
DB03017 Lauric Acid experimental unknown unknown
DB00157 NADH nutraceutical unknown substrate
DB00898 Ethanol approved unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1M6H X-ray 2.0Å A/B=2-374.
1M6W X-ray 2.3Å A/B=2-374.
1MA0 X-ray 2.3Å A/B=2-374.
1MC5 X-ray 2.6Å A/B=1-374.
1MP0 X-ray 2.2Å A/B=2-374.
1TEH X-ray 2.7Å A/B=2-374.
2FZE X-ray 1.9Å A/B=2-374.
2FZW X-ray 1.8Å A/B=2-374.
3QJ5 X-ray 1.9Å A/B=2-374.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00010 Glycolysis / Gluconeogenesis - Homo sapiens (human)
hsa00071 Fatty acid degradation - Homo sapiens (human)
hsa00350 Tyrosine metabolism - Homo sapiens (human)
hsa00830 Retinol metabolism - Homo sapiens (human)
hsa00980 Metabolism of xenobiotics by cytochrome P450 - Homo sapiens (human)
hsa00982 Drug metabolism - cytochrome P450 - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa01200 Carbon metabolism - Homo sapiens (human)
hsa05204 Chemical carcinogenesis - Homo sapiens (human)