Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000030
UniProt IDO00154
Primary gene name(s)ACOT7
Synonym gene name(s)BACH
Protein nameCytosolic acyl coenzyme A thioester hydrolase
Protein functionAcyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A, CoASH, providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. May play an important physiological function in brain. May play a regulatory role by modulating the cellular levels of fatty acyl-CoA ligands for certain transcription factors as well as the substrates for fatty acid metabolizing enzymes, contributing to lipid homeostasis. Has broad specificity, active towards fatty acyl-CoAs with chain-lengths of C8-C18. Has a maximal activity toward palmitoyl-CoA.
Subcellular locationIsoform 4: Cytoplasm.;
SUBCELLULAR LOCATION: Isoform 6: Cytoplasm.;
SUBCELLULAR LOCATION: Isoform 1: Mitochondrion.;
SUBCELLULAR LOCATION: Isoform 5: Mitochondrion.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O00154
Gene Ontology
(Biological Process)
Complete annatation
coenzyme A biosynthetic process [GO:0015937];
long-chain fatty-acyl-CoA biosynthetic process [GO:0035338];
long-chain fatty-acyl-CoA catabolic process [GO:0036116];
medium-chain fatty acid biosynthetic process [GO:0051792];
medium-chain fatty-acyl-CoA catabolic process [GO:0036114];
palmitic acid biosynthetic process [GO:1900535]
Gene Ontology
(Molecular Function)
Complete annatation
acyl-CoA hydrolase activity [GO:0047617];
carboxylic ester hydrolase activity [GO:0052689];
fatty-acyl-CoA binding [GO:0000062];
long-chain fatty acyl-CoA binding [GO:0036042];
palmitoyl-CoA hydrolase activity [GO:0016290];
protein homodimerization activity [GO:0042803]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
mitochondrion [GO:0005739];
nucleoplasm [GO:0005654]
Protein-protein interaction116460
Phylogenetic treeO00154
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD32.170173458280813.08877302290789e-072.68364864727207e-06
AZA vs. DISU0.08186806518861650.7517168649937820.976021571236163
AZA vs. IL70.9089473879476210.0002819547042846040.0324420096006247
AZA vs. SAHA0.05165981982475290.8371509064869930.961440456451378
DISU vs. CD3-2.099915898324188.10514859117539e-078.03796117522223e-06
DISU vs. IL70.8169420949480830.001692867081897980.0381361509195381
DISU vs. SAHA-0.02760880516583890.9257404041902430.982856659937396
DMSO vs. AZA0.1191751098652940.5015733848398981
DMSO vs. CD3-2.062013746543163.85105743627712e-072.97977299833152e-06
DMSO vs. DISU0.03517703984295320.8879565000330890.987802141379617
DMSO vs. IL70.7976386635578620.0003964668848038810.0301855469112046
DMSO vs. SAHA-0.07164534497639130.7683198600587350.933743134500862
HIV vs. Mock in Activation-0.008690727276371710.9920575114034870.999983755607037
HIV vs. Mock in Latency0.01842501027043170.9386990660569880.999834320637052
IL7 vs. CD3-1.256181075083230.003331192257494450.0119010144048422
SAHA vs. CD3-2.141289831579713.43921549006421e-062.45518604619932e-05
SAHA vs. IL7-0.8577140222122530.004258896929662190.0343675654805824
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
2 0.00070511 1.4 0.010039183 1.7 0.001294613
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.45852 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
0.046 5.97E-04

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.023 1.01 1.024 1.03 1.004
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
208002_s_at 2.07 No upregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2QQ2 X-ray 2.8Å A/B/C/D/E/F/G/H/I/J/K/L=209-378.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00062 Fatty acid elongation - Homo sapiens (human)
hsa01040 Biosynthesis of unsaturated fatty acids - Homo sapiens (human)