Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000026
UniProt IDQ9UHC3
Primary gene name(s)ASIC3
Synonym gene name(s)ACCN3, SLNAC1, TNAC1
Protein nameAcid-sensing ion channel 3
Protein functionCation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Generates a biphasic current with a fast inactivating and a slow sustained phase. In sensory neurons is proposed to mediate the pain induced by acidosis that occurs in ischemic, damaged or inflamed tissue. May be involved in hyperalgesia. May play a role in mechanoreception. Heteromeric channel assembly seems to modulate channel properties. {ECO:0000269|PubMed:9744806, ECO:0000269|PubMed:9886053}.
Subcellular locationCell membrane {ECO:0000250};
Multi-pass membrane protein {ECO:0000250}. Cytoplasm {ECO:0000250}. Note=Cell surface expression may be stabilized by interaction with LIN7B and cytoplasmic retention by interaction with DLG4. In part cytoplasmic in cochlea cells, By similarity. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9UHC3
Gene Ontology
(Biological Process)
Complete annatation
detection of chemical stimulus involved in sensory perception of pain [GO:0050968];
detection of mechanical stimulus involved in sensory perception of pain [GO:0050966];
detection of temperature stimulus involved in sensory perception of pain [GO:0050965];
ion transmembrane transport [GO:0034220];
response to acidic pH [GO:0010447];
response to heat [GO:0009408];
sensory perception [GO:0007600];
sensory perception of sour taste [GO:0050915];
signal transduction [GO:0007165];
transport [GO:0006810]
Gene Ontology
(Molecular Function)
Complete annatation
acid-sensing ion channel activity [GO:0044736];
cation channel activity [GO:0005261];
enterobactin transporter activity [GO:0042931];
sodium channel activity [GO:0005272]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
integral component of plasma membrane [GO:0005887];
plasma membrane [GO:0005886]
Protein-protein interaction114723
Phylogenetic treeQ9UHC3
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.3591595660030120.3020823757323870.420637986607403
AZA vs. DISU1.214056071170920.005366655439882020.18859509774453
AZA vs. IL7-0.1821114560093260.5118396335029740.999311006273513
AZA vs. SAHA0.7067410702398940.007361790465391430.0798357046584967
DISU vs. CD31.556640407230760.001503209535519190.00585520707577775
DISU vs. IL7-1.407430756575710.002332697447508970.0471386246932945
DISU vs. SAHA-0.504963497629390.2302920593580080.608046886227145
DMSO vs. AZA0.05596120723049170.8164535664936721
DMSO vs. CD30.4048320638205810.2329271072165350.33659922016471
DMSO vs. DISU-1.160322788337490.008606152646206230.199223840826925
DMSO vs. IL7-0.2305207642903870.4201181805171490.853104173397827
DMSO vs. SAHA0.6445595319003480.01097039084226780.095499502451414
HIV vs. Mock in Activation0.2527195908118090.7022827884341140.999983755607037
HIV vs. Mock in Latency-0.1465265456675870.4916840763753610.999834320637052
IL7 vs. CD30.1783469855999370.6028877909998750.719446415465214
SAHA vs. CD31.041360086856370.005059719110778070.0144221613390251
SAHA vs. IL70.8857461292952430.0009424475379571360.011127591865288
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
NOTEST 0.691659 1
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB08838 Agmatine experimental, investigational yes agonist

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04750 Inflammatory mediator regulation of TRP channels - Homo sapiens (human)
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