Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000025
UniProt IDP78348
Primary gene name(s)ASIC1
Synonym gene name(s)ACCN2, BNAC2
Protein nameAcid-sensing ion channel 1
Protein functionIsoform 2 and isoform 3 function as proton-gated sodium channels; they are activated by a drop of the extracellular pH and then become rapidly desensitized. The channel generates a biphasic current with a fast inactivating and a slow sustained phase. Has high selectivity for sodium ions and can also transport lithium ions with high efficiency. Isoform 2 can also transport potassium, but with lower efficiency. It is nearly impermeable to the larger rubidium and cesium ions. Isoform 3 can also transport calcium ions. Mediates glutamate-independent Ca(2+ entry into neurons upon acidosis. This Ca(2+ overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+ concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear. {ECO:0000269|PubMed:22760635}.; FUNCTION: Isoform 1 does not display proton-gated cation channel activity. {ECO:0000269|PubMed:22760635}.
Subcellular locationCell membrane {ECO:0000269|PubMed:12578970, ECO:0000269|PubMed:21036899, ECO:0000269|PubMed:22760635};
Multi-pass membrane protein {ECO:0000269|PubMed:12578970, ECO:0000269|PubMed:21036899, ECO:0000269|PubMed:22760635}. Note=Localizes in synaptosomes at dendritic synapses of neurons. Colocalizes with DLG4, By similarity. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P78348
Gene Ontology
(Biological Process)
Complete annatation
associative learning [GO:0008306];
calcium ion transmembrane transport [GO:0070588];
cellular response to pH [GO:0071467];
ion transmembrane transport [GO:0034220];
memory [GO:0007613];
negative regulation of neurotransmitter secretion [GO:0046929];
protein homotrimerization [GO:0070207];
regulation of membrane potential [GO:0042391];
response to acidic pH [GO:0010447];
response to pH [GO:0009268];
sensory perception of sour taste [GO:0050915];
signal transduction [GO:0007165];
sodium ion transmembrane transport [GO:0035725];
sodium ion transport [GO:0006814];
transport [GO:0006810]
Gene Ontology
(Molecular Function)
Complete annatation
acid-sensing ion channel activity [GO:0044736];
ion gated channel activity [GO:0022839];
ligand-gated sodium channel activity [GO:0015280]
Gene Ontology
(Cellular Component)
Complete annatation
cell surface [GO:0009986];
integral component of plasma membrane [GO:0005887];
plasma membrane [GO:0005886];
synapse [GO:0045202]
Protein-protein interaction106559
Phylogenetic treeP78348
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.6715129700396930.04677422050237510.0944635296410784
AZA vs. DISU-0.7006961258463610.007817510780125820.221590064552732
AZA vs. IL70.06514681158228920.7896832323627760.999311006273513
AZA vs. SAHA0.1091662001278170.6654249399357860.900627283556383
DISU vs. CD3-0.04291693879364890.9075758025742830.939744308833957
DISU vs. IL70.7588452272565590.003734286643298670.0624106110285226
DISU vs. SAHA0.8101099292549780.006986305239674470.0813054874649683
DMSO vs. AZA0.07073689014942980.799360700356171
DMSO vs. CD30.7205227937681480.02885133319975690.0607980605195604
DMSO vs. DISU0.7668537089474940.006271838594486770.169760733783758
DMSO vs. IL70.003449677332435890.9908348888860680.998901777988831
DMSO vs. SAHA0.03366784571346130.9035543821458220.977068323939473
HIV vs. Mock in Activation-0.04127966131633060.9482309650037260.999983755607037
HIV vs. Mock in Latency-0.5990343868624950.0006397084683069650.0432419975392789
IL7 vs. CD30.7475753978500080.02380979889819310.0601752345393134
SAHA vs. CD30.7540669601212620.03678853590541520.0770004494575843
SAHA vs. IL70.03735059060299540.8816887773917120.953237115775459
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
0.3618 0.04208

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.204484 0.351458
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00594 Amiloride approved unknown inhibitor
DB00586 Diclofenac approved, vet_approved yes inhibitor

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04750 Inflammatory mediator regulation of TRP channels - Homo sapiens (human)