Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0000012
UniProt IDP61221
Primary gene name(s)ABCE1
Synonym gene name(s)RLI, RNASEL1, RNASELI, RNS4I
Protein nameATP-binding cassette sub-family E member 1
Protein functionAntagonizes the binding of 2-5A, 5'-phosphorylated 2',5'-linked oligoadenylates by RNase L through direct interaction with RNase L and therefore inhibits its endoribonuclease activity. May play a central role in the regulation of mRNA turnover. Antagonizes the anti-viral effect of the interferon-regulated 2-5A/RNase L pathway. May act as a chaperone for post-translational events during HIV-1 capsid assembly. {ECO:0000269|PubMed:11585831, ECO:0000269|PubMed:9660177, ECO:0000269|PubMed:9847332}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:11585831}. Mitochondrion {ECO:0000269|PubMed:11585831}. Note=Localized to clusters of virus formation at the plasma membrane.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P61221
Gene Ontology
(Biological Process)
Complete annatation
negative regulation of endoribonuclease activity [GO:0060702];
ribosomal subunit export from nucleus [GO:0000054];
translational initiation [GO:0006413];
translational termination [GO:0006415];
transmembrane transport [GO:0055085];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation
ATPase activity [GO:0016887];
ATP binding [GO:0005524];
ribonuclease inhibitor activity [GO:0008428]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
membrane [GO:0016020];
mitochondrial matrix [GO:0005759];
mitochondrion [GO:0005739]
Protein-protein interaction111986
Phylogenetic treeP61221
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD32.13955513563563.44037132116171e-105.41831520925279e-09
AZA vs. DISU-0.2057475078188730.4155508195676190.911012587516386
AZA vs. IL70.5367733027823570.02523651702972550.507863846714356
AZA vs. SAHA-0.4226949122840670.08334071299993550.360306045615334
DISU vs. CD3-2.35760445436445.59396085009212e-101.19777508315751e-08
DISU vs. IL70.7340254477269440.003686119865890070.0619346157167016
DISU vs. SAHA-0.2168619025918320.4568132308251210.796740215457168
DMSO vs. AZA-0.06789685975155380.6879407776369931
DMSO vs. CD3-2.219582303755512.91211499359179e-115.00239593825088e-10
DMSO vs. DISU0.1358126919191990.577265670115210.932341451680097
DMSO vs. IL70.6120614009132480.004831129248861870.14059705294709
DMSO vs. SAHA-0.3622315021796670.1245980246989790.421426725271243
HIV vs. Mock in Activation-0.2085195391322910.7379359908525890.999983755607037
HIV vs. Mock in Latency-0.06596556901788670.6892713450551240.999834320637052
IL7 vs. CD3-1.593220482544871.22167909410997e-061.1691662847949e-05
SAHA vs. CD3-2.587650708049484.76374495406162e-121.28886241396245e-10
SAHA vs. IL7-0.963673245939978.68772926078121e-050.00174572781957711
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.390615 0.00241278
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.012 1.015 0.914 0.811 0.892
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Pr55(Gag) co-localizes with 17233757
Pr55(Gag) associates with 11780123
Pr55(Gag) regulated by 17222823
Vif associates with 11780123
Pr55(Gag) interacts with 16275648

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found